ERK2 Antibody - BSA Free

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebellum shows moderate cytoplasmic positivity in Purkinje cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human cerebral cortex shows weak cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: ERK2 Antibody [NBP1-87943] - Staining of human pancreas shows very weak cytoplasmic positivity in exocrine glandular cells.

Simple Western: ERK2 Antibody [NBP1-87943] - Simple Western lane view shows a specific band for ERK2 in 0.2 mg/ml of U-2 OS (left) and h. Pancreas (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: ERK2 Antibody [NBP1-87943] - Electropherogram image of the corresponding Simple Western lane view. ERK2 antibody was used at 1:5 dilution on U-2 OS and h. Pancreas lysate(s) respectively.

Analysis in human cell line HDLM-2.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ERK2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: YIVQDLMETDLYKLLKTQHLSNDHICY
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Simple Western 1:5
  • Western Blot 1:100-1:250
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. In Simple Western only 10-15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in U-2 OS and h pancreas lysates, separated by Size, antibody dilution of 1:5
• Control Peptide:

  ERK2 Recombinant Protein Antigen (NBP1-87943PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ERK2 Antibody - BSA Free

• EC 2.7.11
• EC 2.7.11.24
• ERK
• ERK2
• ERK-2
• ERK2MAP kinase isoform p42
• ERT1
• Extracellular signal-regulated kinase 2
• MAP kinase 1
• MAP kinase 2
• MAPK 1
• MAPK1
• MAPK2
• mitogen-activated protein kinase 1
• Mitogen-activated protein kinase 2
• p38
• p40
• p41
• p41mapk
• p42mapk
• p42-MAPK
• PRKM1
• PRKM1MAPK 2
• PRKM2
• protein tyrosine kinase ERK2

Background

Extracellular signal regulated kinase 2 (ERK 2) also known as maturation or mitogen

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK1