ERK1 [p Thr202, p Tyr204] Antibody - Azide Free

Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho Thr202/Tyr204 ERK/MAP antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei (blue). Photo courtesy of Robert Wine.

• Reactivity: Hu, Mu, Rt, Ch, Bv, Ca, Ch, Pm, Xp, Ze
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

ERK1 [p Thr202, p Tyr204] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit ERK1 [p Thr202, p Tyr204] Antibody - Azide Free (NB500-141) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ERK1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding ERK1 conjugated to KLH. Accession # P63086
• Modification: p Thr202, p Tyr204
• Localization: Cell Membrane
• Specificity: Specific for endogenous levels of the ~42 - 44 kDa ERK1 protein phosphorylated at Thr202 and Tyr204. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Zebrafish (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK3
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100 - 1:250
  • Immunohistochemistry 1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000
• Application Notes: NB500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26232338)
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken reactivity reported in scientific literature (PMID: 31400551).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for ERK1 [p Thr202, p Tyr204] Antibody - Azide Free

• EC 2.7.11
• ERK1
• ERK-1
• ERK1p44-MAPK
• ERT2
• Extracellular signal-regulated kinase 1
• extracellular signal-related kinase 1
• HS44KDAP
• HUMKER1A
• Insulin-stimulated MAP2 kinase
• MAP kinase 1
• MAP kinase 3
• MAP kinase isoform p44
• MAPK 1
• MAPK 3
• MAPK3
• MGC20180
• Microtubule-associated protein 2 kinase
• Mitogen-activated protein kinase 1
• mitogen-activated protein kinase 3
• P44ERK1
• p44-ERK1
• p44mapk
• PRKM3
• PRKM3EC 2.7.11.24

Background

The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at Tyrosine 204 and 187 and Threonine 177 and 160 residues mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both the Threonine 202 and Tyrosine 204 residues of ERK 1 and Threonine 185 and Tyrosine 187 residues of ERK 2 is required for full enzymatic activation. The structural consequences of dual phosphorylation in ERK 2 include active site closure, alignment of key catalytic residues that interact with ATP, and remodeling of the activation loop. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERK1 Antibody (NB500-141)(3)

Publications using NB500-141

• Wang N, Lu L, Cao Qf Et Al. Partial inhibition of activin receptor-like kinase 4 alleviates bladder fibrosis caused by bladder outlet obstruction Experimental cell research 2021-07-06 [PMID: 34237300] (WB)
• Lin X, Ma Y, Qian T et al. Basic Fibroblast Growth Factor Promotes Prehierarchical Follicle Growth and Yolk Deposition in the Chicken Theriogenology 2019-07-01 [PMID: 31400551] (Chicken)
• Nymoen DA, Hetland Falkenthal TE, Holth A et al. Expression and clinical role of chemoresponse-associated genes in ovarian serous carcinoma. Gynecol. Oncol. 2015-07-29 [PMID: 26232338] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MAPK3
• Entrez:
  • 5595(Human)
  • 26417(Mouse)
  • 50689(Rat)
• Uniprot:
  • P21708(Rat)
  • P21708(Rat)