Western Blot: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Human T47D cell lysates showing specific immunolabeling of ~42-44k ERK/MAPK protein phosphorylated at Thr202/Tyr204 (Control). Phosphospecificity is shown ...read more
Immunohistochemistry: ERK1 [p Thr202, p Tyr204] Antibody [NB500-141] - Immunostaining of granule cells in the dentate gyrus of saline treated mouse showing ERK/MAPK when phosphorylated at Thr202/Tyr204 (red) and nuclei ...read more
Hu, Mu, Rt, Bv, Ca, Ch, Pm, Xp, ZeSpecies Glossary
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH
p Thr202, p Tyr204
Specific for the ~42k - 44k ERK/MAPK phosphorylated at Thr202 and Tyr204. Immunolabeling is blocked by the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. The immunolabeling is completely eliminated by lambda-phosphatase.
NB500-141 can be used in Western blot where a band is seen at ~ 42-44 kDa, representing ERK 1/2. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 26232338)
42 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publication using NB500-141 in the following applications:
Alternate Names for ERK1 [p Thr202, p Tyr204] Antibody
Extracellular signal-regulated kinase 1
extracellular signal-related kinase 1
Insulin-stimulated MAP2 kinase
MAP kinase 1
MAP kinase 3
MAP kinase isoform p44
Microtubule-associated protein 2 kinase
Mitogen-activated protein kinase 1
mitogen-activated protein kinase 3
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases (p44 and p42), designated ERK 1 (extracellular-signal related kinase 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites, which is required for full enzymatic activation. Once activated, MAP kinases phosphorylate downstream components on serine and threonine. Upstream MAP kinase regulators include MEK and Raf-1.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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