ERK1 Antibody (1A2)


Western Blot: ERK1 Antibody (AT1A2) [NBP1-74032] - SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 (1:500). To determine specificity of anti-human MAPK3 antibody, western blot was performed using more
Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (AT1A2) [NBP1-74032] - Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with more

Product Details

Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF
1 mg/ml

ERK1 Antibody (1A2) Summary

Recombinant human MAPK3 protein (1-137aa) purified from E. coli
Anti-human MAPK3 mAb, clone 1A2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAPK3 protein.
IgG2b Kappa
Protein G purified
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 10% Glycerol
0.02% Sodium Azide
1 mg/ml
Protein G purified


  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
The antibody has been tested by ELISA, Western blot, and Immunofluorescence to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500

Reactivity Notes


Alternate Names for ERK1 Antibody (1A2)

  • EC 2.7.11
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MAPK3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • p44erk1
  • p44-ERK1
  • p44mapk
  • PRKM3


Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK1 Antibody (NBP1-74032) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor (R) 405 Labeled NBP1-74032AF405
Alexa Fluor (R) 488 Labeled NBP1-74032AF488
Alexa Fluor (R) 647 Labeled NBP1-74032AF647
Alexa Fluor (R) 700 Labeled NBP1-74032AF700
Allophycocyanin Labeled NBP1-74032APC
DyLight 350 Labeled NBP1-74032UV
DyLight 405 Labeled NBP1-74032V
DyLight 405LS Labeled NBP1-74032V3
DyLight 488 Labeled NBP1-74032G
DyLight 680 Labeled NBP1-74032FR
DyLight 755 Labeled NBP1-74032IR
PE Labeled NBP1-74032PE
PerCP Labeled NBP1-74032PCP

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Blogs on ERK1.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

Contact Information

Product PDFs


Gene Symbol MAPK3

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