ERK1 Antibody (1A2)

Images

 
Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human MAPK3 antibody (1:500). Proteins were visualized using a goat ...read more
Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:100) with goat ...read more
Flow Cytometry: ERK1 Antibody (1A2) [NBP1-74032] - Analysis of MAPK3 in HeLa cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
Western Blot: ERK1 Antibody (1A2) [NBP1-74032] - The Recombinant proteins (50ng) were resolved by SDSPAGE, transferred to PVDF membrane and probed with antihuman MAPK3 antibody (1:500). Proteins were visualized using a ...read more
Immunocytochemistry/ Immunofluorescence: ERK1 Antibody (AT1A2) [NBP1-74032] - Immunofluorescence of HeLa cells stained with Hoechst 3342 (Blue) for nucleus staining and monoclonal anti-human MAPK3 antibody (1:500) with ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
1A2
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

ERK1 Antibody (1A2) Summary

Immunogen
Recombinant human MAPK3 protein (1-137aa) purified from E. coli
Specificity
Anti-human MAPK3 mAb, clone 1A2, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human MAPK3 protein.
Isotype
IgG2b Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MAPK3
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
The antibody has been tested by ELISA, Western blot, and Immunofluorescence to assure specificity and reactivity. Since applications vary, however, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Western blot analysis and Immunofluorescence is 1:250 ~ 500. Recommended starting dilution is 1:500

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for ERK1 Antibody (1A2)

  • EC 2.7.11
  • ERK1
  • ERK-1
  • ERK1p44-MAPK
  • ERT2
  • Extracellular signal-regulated kinase 1
  • extracellular signal-related kinase 1
  • HS44KDAP
  • HUMKER1A
  • Insulin-stimulated MAP2 kinase
  • MAP kinase 1
  • MAP kinase 3
  • MAP kinase isoform p44
  • MAPK 1
  • MAPK 3
  • MAPK3
  • MGC20180
  • Microtubule-associated protein 2 kinase
  • Mitogen-activated protein kinase 1
  • mitogen-activated protein kinase 3
  • P44ERK1
  • p44-ERK1
  • p44mapk
  • PRKM3
  • PRKM3EC 2.7.11.24

Background

Mitogen-activated protein kinase (MAPKs) are a widely conserved family of serine/threonine kinase involved in many cellular programs such as cell proliferation, differentiation, motility, and death. MAPK3 (ERK1) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines and is on important target in the diagnosis and treatment of cancer. Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ERK1 Antibody (NBP1-74032) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-74032AF405
Alexa Fluor 488 Labeled NBP1-74032AF488
Alexa Fluor 647 Labeled NBP1-74032AF647
Alexa Fluor 700 Labeled NBP1-74032AF700
DyLight 350 Labeled NBP1-74032UV
DyLight 405 Labeled NBP1-74032V
DyLight 488 Labeled NBP1-74032G
DyLight 680 Labeled NBP1-74032FR
DyLight 755 Labeled NBP1-74032IR

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Bioinformatics Tool for ERK1 Antibody (NBP1-74032)

Discover related pathways, diseases and genes to ERK1 Antibody (NBP1-74032). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for ERK1 Antibody (NBP1-74032)

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Pathways for ERK1 Antibody (NBP1-74032)

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PTMs for ERK1 Antibody (NBP1-74032)

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Research Areas for ERK1 Antibody (NBP1-74032)

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Blogs on ERK1.

The role of c-Fos in the regulation of the JC virus gene transcription
c-Fos is a member of the AP-1 transcription factor family under the Fos protein family umbrella, alongside Fra-1, Fra-2 and Fos-B.  Also in the AP-1 transcription family are the Jun proteins, c-Jun, Jun-B and Jun-D.  Each member of the AP-1 transcri...  Read full blog post.

The effects of curcumin on IKB Alpha and the NFkB signaling pathway
The IKK complex, or inhibitor of NFkB kinase, is composed of IKK alpha and IKK beta.  These kinases are at the core of the NFkB signaling cascade.  The NFkB family is made up of transcription factors that are kept inactive in the cytoplasm through...  Read full blog post.

MAPK3/ERK1 - A signal transduction pathway with roles in development and disease
Mitogen-activated protein kinases (MAPKs) are important signaling proteins needed to transmit and relay extracellular stimuli and to illicit intracellular responses (1). The MAPK family of proteins are serine/threonine kinases that are able to phos...  Read full blog post.

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Bioinformatics

Gene Symbol MAPK3
Entrez
Uniprot