eRF1 Antibody - BSA Free

Western Blot: eRF1 Antibody [NBP1-31144] - Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE eRF1 antibody, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: eRF1 Antibody [NBP1-31144] - Analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31144] - Sample: Paraffin-embedded mouse brain. eRF1 antibody diluted at 1:500.

Immunohistochemistry: eRF1 Antibody [NBP1-31144] - eRF1 antibody detects eRF1 protein on myofiber membranes by immunohistochemical analysis Sample: Agarose-embedded zebrafish embryo eRF1 antibody (NBP1-31144) dilution: 1:200. Image provided with permission courtesy of Dr. T. Schilling at UC, Irvine.

• Reactivity: Hu, Mu, Rt, Bv, Ca, Rb, RM, Xp
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

eRF1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit eRF1 Antibody - BSA Free (NBP1-31144) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human eRF1. The exact sequence is proprietary.
• Localization: Cytoplasm
• Predicted Species: Rat (100%), Canine (100%), Xenopus (100%), Rhesus Macaque (100%), Bovine (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ETF1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for eRF1 Antibody - BSA Free

• D5S1995
• ERF1eRF1
• eukaryotic peptide chain release factor subunit 1
• Eukaryotic release factor 1
• eukaryotic translation termination factor 1
• polypeptide chain release factor 1
• Protein Cl1
• RF1ERF
• sup45 (yeast omnipotent suppressor 45) homolog-like 1
• SUP45L1
• TB3-1MGC111066

Background

Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ETF1