eRF1 Antibody


Western Blot: eRF1 Antibody [NBP1-31113] - Sample (30 ug of whole cell lysate) A: Molt-4 10% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: eRF1 Antibody [NBP1-31113] - Analysis of HeLa, using eRF1 antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: eRF1 Antibody [NBP1-31113] - Paraffin-embedded SW480 xenograft, using antibody at 1:100 dilution.

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, DB, ICC/IF, IHC, IHC-P
0.91 mg/ml

Order Details

eRF1 Antibody Summary

Synthetic peptide contain a sequence corresponding to a region within amino acids 374 and 387 (P62495) of eRF1
Predicted Species
Rat (100%), Bovine (100%), Primate (100%), Rabbit (100%), Xenopus (93%), Canine (100%)
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7.0).
0.01% Thimerosal
0.91 mg/ml
Immunogen affinity purified


Application Notes
Dot blot, Western blot, Immunohistochemistry-Paraffin and Immunofluorescence. The usefulness of this product in other applications has not been determined.

Reactivity Notes

Tested in human. Predicted cross-reactivity based on sequence identity: Xenopus laevis (93%), Xenopus Tropicalis (100%), and Rhesus Monkey (100%). Not yet tested in any other species.

Alternate Names for eRF1 Antibody

  • D5S1995
  • ERF1eRF1
  • eukaryotic peptide chain release factor subunit 1
  • Eukaryotic release factor 1
  • eukaryotic translation termination factor 1
  • polypeptide chain release factor 1
  • Protein Cl1
  • RF1ERF
  • sup45 (yeast omnipotent suppressor 45) homolog-like 1
  • SUP45L1
  • TB3-1MGC111066


Termination of protein biosynthesis and release of the nascent polypeptide chain are signaled by the presence of an in-frame stop codon at the aminoacyl site of the ribosome. The process of translation termination is universal and is mediated by protein release factors (RFs) and GTP. A class 1 RF recognizes the stop codon and promotes the hydrolysis of the ester bond linking the polypeptide chain with the peptidyl site tRNA, a reaction catalyzed at the peptidyl transferase center of the ribosome. Class 2 RFs, which are not codon specific and do not recognize codons, stimulate class 1 RF activity and confer GTP dependency upon the process. In prokaryotes, both class 1 RFs, RF1 and RF2, recognize UAA; however, UAG and UGA are decoded specifically by RF1 and RF2, respectively. In eukaryotes, eRF1, or ETF1, the functional counterpart of RF1 and RF2, functions as an omnipotent RF, decoding all 3 stop codons (Frolova et al., 1994 [PubMed 7990965]).[supplied by OMIM]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies


Isotype Controls

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Contact Information

Product PDFs


Gene Symbol ETF1

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