Erbin Antibody [Unconjugated]

Images

 
Western blot shows lysates of A172 human glioblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Erbin Antigen Affinity-purified ...read more
Erbin was detected in immersion fixed DU145 human prostate carcinoma cell line using Sheep Anti-Human Erbin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7866) at 10 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Erbin Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Erbin
Lys496-Thr687
Accession # Q96RT1
Specificity
Detects human Erbin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse ErbB2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
ERBB2IP
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Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF7866 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erbin Antibody [Unconjugated]

  • Densin-180-like protein
  • erbb2 interacting protein
  • Erbb2-interacting proteinErbin
  • ERBB2IP
  • Erbin
  • ERBINLAP2protein LAP2
  • KIAA1225

Background

ErbB2IP (ErbB2 Interacting Protein/Erbin; also Densin-180-like protein and LAP2) is both a nuclear and cytoplasmic 175-180 kDa member of the LAP (LRR And PDZ) family of proteins. It is widely expressed, and found in cell types as diverse as intestinal columnar epithelium, neurons, keratinocytes and skeletal plus cardiac muscle cells. Functionally, ErbB2IP interacts with multiple proteins through a PZD-mediated interaction. Partners include ErbB2, δ-catenin, PSD-95 and ARVCF. The ErbB2 interaction localizes this receptor to the basolateral membrane of epithelium, while the δ-catenin interaction impacts dendritic arborization, possibly through involvement with type 1 or 2 catenin(s) and the F-actin cytoskeleton. Finally, ErbB2IP/Erbin is suggested to play a role in maintaining the G1 phase of the cell cycle. Human ErbB2IP is 1412 amino acids (aa) in length. It contains 17 LRRs (Leu-rich repeats) (aa 23-413) followed by one PDZ domain (aa 1321-1410). There are more than 10 utilized Tyr, and 15 utilized Ser/Thr phosphorylation sites. A series of deletions over aa 1211-1377 generate at least six known splice variants. All show a deletion of aa 1212-1252, and at least one additional 10-41 aa deletion C-terminal to the common deletion. Over aa 496-687, human and mouse ErbB2IP share 88% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Reviews for Erbin Antibody (AF7866) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Pig.

Reviews using AF7866:
Filter by Applications
ICC
(2)
All Applications
Filter by Species
Pig
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry Erbin AF7866
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reviewed by:
Anonymous
ICC Pig 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig SC cells
SpeciesPig
LotN/A
Immunocytochemistry Erbin AF7866
Enlarge
reviewed by:
Anonymous
ICC Pig 06/07/2017
View

Summary

ApplicationImmunocytochemistry
Sample Testedpig trabecular meshwork cells
SpeciesPig
LotN/A

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Recent Reviews

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Anonymous
06/07/2017
Application: ICC
Species: Pig

Anonymous
06/07/2017
Application: ICC
Species: Pig

Bioinformatics

Gene Symbol ERBB2IP
Uniprot
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