Erbin Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 700

Erbin Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Erbin
  Lys496-Thr687
  Accession # Q96RT1
• Specificity: Detects human Erbin in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant mouse ErbB2 is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Erbin Antibody [Alexa Fluor® 700]

• Densin-180-like protein
• erbb2 interacting protein
• Erbb2-interacting proteinErbin
• ERBB2IP
• Erbin
• ERBINLAP2protein LAP2
• KIAA1225

Background

ErbB2IP (ErbB2 Interacting Protein/Erbin; also Densin-180-like protein and LAP2) is both a nuclear and cytoplasmic 175-180 kDa member of the LAP (LRR And PDZ) family of proteins. It is widely expressed, and found in cell types as diverse as intestinal columnar epithelium, neurons, keratinocytes and skeletal plus cardiac muscle cells. Functionally, ErbB2IP interacts with multiple proteins through a PZD-mediated interaction. Partners include ErbB2, δ-catenin, PSD-95 and ARVCF. The ErbB2 interaction localizes this receptor to the basolateral membrane of epithelium, while the δ-catenin interaction impacts dendritic arborization, possibly through involvement with type 1 or 2 catenin(s) and the F-actin cytoskeleton. Finally, ErbB2IP/Erbin is suggested to play a role in maintaining the G1 phase of the cell cycle. Human ErbB2IP is 1412 amino acids (aa) in length. It contains 17 LRRs (Leu-rich repeats) (aa 23-413) followed by one PDZ domain (aa 1321-1410). There are more than 10 utilized Tyr, and 15 utilized Ser/Thr phosphorylation sites. A series of deletions over aa 1211-1377 generate at least six known splice variants. All show a deletion of aa 1212-1252, and at least one additional 10-41 aa deletion C-terminal to the common deletion. Over aa 496-687, human and mouse ErbB2IP share 88% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.