ErbB2/Her2 Antibody (H2M5B) [Unconjugated]

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MCF-7 human cell line was stained with Mouse Anti-Human ErbB2/Her2 Monoclonal Antibody (Catalog # MAB9896, filled histogram) or Mouse IgG1 isotype control (Catalog # MAB002, open histogram) followed by anti-Mouse IgG ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, CyTOF-ready
Clone
H2M5B
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

ErbB2/Her2 Antibody (H2M5B) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human ErbB2/Her2
Specificity
Detects human ErbB2/Her2 in direct ELISAs based on the variable region sequence of Trastuzumab, using a mouse IgG1 backbone structure.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • CyTOF-ready
Positive Control
ErbB2/Her2 Lysate (NBP2-64719)

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB2/Her2 Antibody (H2M5B) [Unconjugated]

  • CD340 antigen
  • CD340
  • c-erb B2/neu protein
  • EC 2.7.10
  • EGFR2
  • ErbB2
  • HER2
  • HER-2
  • HER2EC 2.7.10.1
  • herstatin
  • Metastatic lymph node gene 19 protein
  • MLN 19
  • MLN19
  • Neu Oncogene
  • NEUHER-2/neu
  • neuroblastoma/glioblastoma derived oncogene homolog
  • NGL
  • NGLTKR1
  • p185erbB2
  • Proto-oncogene c-ErbB-2
  • Proto-oncogene Neu
  • receptor tyrosine-protein kinase erbB-2
  • TKR1
  • Tyrosine kinase-type cell surface receptor HER2
  • v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog)
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Background

Trastuzumab is a biosimilar directed against the extracellular domain of Her2 (ErbB2). This product uses the variable region sequence of Trastuzumab and has replaced the human IgG1 backbone structure with mouse IgG1. This product can be used as the standard in pharmacokinetic (PK) assays.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for ErbB2/Her2 Antibody (MAB9896). (Showing 1 - 1 of 1 FAQ).

  1. I am interested in getting antibodies for HER that would work in flow cytometry. Could you recommend some choices?
    • A list of our ErbB2 antibodies validated for use in flow cytometry can be found here /productsearch/HER2#fq=common_name%3A%22ErbB2%2FHER2%22&fq=category%3A%22Primary%20Antibodies%22&fq=applications%3A%22Flow%20Cytometry%22

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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