ERAB Antibody

Western Blot: ERAB Antibody [NB300-887] - Analysis of ERAB / HADH2 in Human Brain lysate (RIPA buffer, 30ug total protein per lane) using this antibody at 0.3ug/ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, PEP-ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ERAB Antibody Summary

• Immunogen: Peptide with sequence CIRLDGAIRMQP corresponding to C-Terminus according to NP_004484.1, NP_001032900.1.
• Specificity: This antibody is expected to recognise both reported isoforms.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: HSD17B10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.3 - 2 ug/ml
  • Peptide ELISA Detection limit 1:32000
• Application Notes: WB: Approx. 25 kDa band observed in human brain lysates (calculated MW of 26.9 kDa band according to NP_004484). A minor band of unknown identity was also consistently observed at appr 38 kDa. This band was successfully blocked by incubation with the immunizing peptide.
• Positive Control:

  ERAB Lysate (NBL1-11722)

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine. Mouse reactivity determined by Western Blot reported in scientific literature (PMID: 17436268).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ERAB Antibody

• 17-beta-HSD 10
• 17-beta-hydroxysteroid dehydrogenase 10
• 17b-HSD10
• 3-hydroxy-2-methylbutyryl-CoA dehydrogenase
• ABAD
• amyloid-beta peptide binding alcohol dehydrogenase
• CAMR
• DUPXp11.22
• EC 1.1.1.178
• EC 1.1.1.35
• Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
• ERAB3-hydroxyacyl-CoA dehydrogenase type-2
• HADH2AB-binding alcohol dehydrogenase
• hydroxyacyl-Coenzyme A dehydrogenase, type II, hydroxyacyl-Coenzyme Adehydrogenase, type II
• hydroxysteroid (17-beta) dehydrogenase 10
• mental retardation, X-linked, syndromic 10
• MHBD
• Mitochondrial ribonuclease P protein 2,3-hydroxyacyl-CoA dehydrogenase type II
• Mitochondrial RNase P protein 2
• MRPP2HCD2
• MRX17
• MRX31
• MRXS10
• SCHAD
• SDR5C1
• short chain dehydrogenase/reductase family 5C, member 1
• short chain L-3-hydroxyacyl-CoA dehydrogenase type 2
• short chain type dehydrogenase/reductase XH98G2
• Short-chain type dehydrogenase/reductase XH98G2
• Type II HADH
• XH98G2

Background

This gene encodes 3-hydroxyacyl-CoA dehydrogenase type II, a member of the short-chain dehydrogenase/reductase superfamily. The gene product is a mitochondrial protein that catalyzes the oxidation of a wide variety of fatty acids, alcohols, and steroids. The protein has been implicated in the development of Alzheimer's disease, and mutations in the gene are the cause of 2-methyl-3-hydroxybutyryl-CoA dehydrogenase deficiency (MHBD). Several alternatively spliced transcript variants have been identified, but the full-length nature of only two transcript variants has been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ERAB Antibody (NB300-887)(2)

Publications using NB300-887

• Tilton RG, Haidacher SJ, Lejeune WS et al. Diabetes-induced changes in the renal cortical proteome assessed with two-dimensional gel electrophoresis and mass spectrometry. Proteomics. 2007 May [PMID: 17436268] (WB, Mouse)
  
  Details:
  WB (mouse tissue lysates), Fig. 4.
• He XY, Schulz H, Yang SY. A human brain L-3-hydroxyacyl-coenzyme A dehydrogenase is identical to an amyloid beta-peptide-binding protein involved in Alzheimer's disease. J Biol Chem 1998 Apr 24 [PMID: 9553139]

Bioinformatics

• Gene Symbol: HSD17B10
• Entrez:
  • 3028(Human)
  • 15108(Mouse)
  • 63864(Rat)
• Uniprot:
  • NP_004484.(Human)