Eps8 Antibody

Images

 
Immunohistochemistry-Paraffin: Eps8 Antibody [NB300-819] - (5ug/ml) staining of paraffin embedded Human Breast Carcinoma. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Eps8 Antibody Summary

Immunogen
Peptide with sequence C-SGVESFDEGSSH corresponding to C-Terminus according to NP_004438.3.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPS8
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5 - 10 ug/ml
  • Immunohistochemistry-Paraffin 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:8000
Application Notes
WB: Preliminary experiments gave no signal but low background in A431 and Hela lysates at up to 1 ug/ml. IHC-P: Human breast Carcinoma shows staining of cancer cells infiltrated in a breast lobe.
Positive Control
Eps8 Lysate (NBL1-10303)
Publications
Read Publication using NB300-819.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Porcine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Eps8 Antibody

  • epidermal growth factor receptor kinase substrate 8
  • epidermal growth factor receptor pathway substrate 8
  • Eps8

Background

This gene encodes a member of the EPS8 family. This protein contains one PH domain and one SH3 domain. It functions as part of the EGFR pathway, though its exact role has not been determined. Highly similar proteins in other organisms are involved in the transduction of signals from Ras to Rac and growth factor-mediated actin remodeling. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Eps8 Antibody (NB300-819)(1)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on Eps8.

Hsc70 - a chaperone protein with diverse cellular functions
Heat shock cognate 71 kDa protein (Hsc70), also known as HSPA8, is a member of the heat shock protein 70 family (Hsp70). Unlike Hsp70, it is a constitutively expressed chaperone protein and is involved in diverse cellular processes including protei...  Read full blog post.

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Bioinformatics

Gene Symbol EPS8
Entrez
Uniprot