USP6NL Antibody

Western Blot: USP6NL Antibody [NBP2-20839] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

USP6NL Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human USP6NL. The exact sequence is proprietary.
• Predicted Species: Mouse (94%), Rat (93%), Rhesus Macaque (99%), Bovine (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: USP6NL
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 94 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for USP6NL Antibody

• KIAA0019RNTRErelated to the N terminus of tre
• Related to the N-terminus of tre
• RN-tre
• TRE2NL
• USP6 N-terminal like
• USP6 N-terminal-like protein

Background

Acts as a GTPase-activating protein for RAB5A. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Review for USP6NL Antibody (NBP2-20839) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/15/2017

Summary

• Application: Western Blot
• Sample Tested: liver and primary hepatocytes
• Species: Human
• Lot: 40499

Comments

• Comments: human liver and primary hepatocytes
  Name: Anti-USP6NL antibody (NBP2-20839)
  Catalog #: Anti-USP6NL antibody (NBP2-20839)
  Lot Number: Anti-USP6NL antibody (NBP2-20839, Lot # 40499)
  PO/Order Number: Click here to enter text..
  WB Image Description (Please provide labels for all lanes): lane 1: human liver; lane 2: human primary hepatocytes
  Sample Information:
       Cell Line or Tissue: human liver, and human primary hepatocytes
       Species: human
       Treatment: No treatment
  Lysate Preparation:
       Date of lysate preparation: December 15, 2017
       Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
  Reducing agent: beta-mercaptoethanol, DTT
  If boiled (temperature/time): Yes
  Controls:
       Positive Control: No
       Negative Control: No
       Loading Control (please attach additional images if applicable): No
  Protein Amount Loaded per lane: 20 ug
  Antibody Storage Conditions: -20℃
  Electrophoresis:
  Gel Percentage: 15%
  Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
       Voltage: 120V
       Time: 2 hours
  Membrane Transfer:
       Method (Submersion/Semi-dry): wet transfer
       Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
       Time: 2 hours
       Voltage: 100V
  Blocking:
       Blocking Solution: 5% milk in 1X TBST
       Time: 1 hour at room temperature
  Primary Antibody:
       Dilution: 1/5000
       Diluent Buffer: 2.5% BSA
       Incubation Time: overnight
       Incubation Temperature: 4℃
  Washing Conditions:
       Wash Solution: 1X TBST
       Time and Repetitions: 5 min each for 3 times
  Secondary Antibody
  Manufacturer and Catalog #: Promega, W401B, Lot # 0000187662
  Secondary description: goat anti-rabbit secondary antibody
       Dilution: 1/2000
       Diluent Buffer: 3% milk
       Incubation Time: 1 hour
       Incubation Temperature: room temperature
  Detection Method:
  Detection: ECL (GE, cat # RPN2209, lot # 9838243)
  Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
  Development Time: 20 seconds
  Molecular weight of band(s): ~ 97 kDa
  Experimental Concerns and Observations: A specific band around 97 kDa was observed

Bioinformatics

• Gene Symbol: USP6NL
• Uniprot:
  • Q92738(Human)