USP6NL Antibody

Images

 
Western Blot: USP6NL Antibody [NBP2-20839] - Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

USP6NL Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human USP6NL. The exact sequence is proprietary.
Predicted Species
Mouse (94%), Rat (93%), Bovine (97%), Rhesus Macaque (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
USP6NL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
94 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP2-20839 in the following application:

Reactivity Notes

Chicken (84%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Immunogen affinity purified

Alternate Names for USP6NL Antibody

  • KIAA0019RNTRErelated to the N terminus of tre
  • Related to the N-terminus of tre
  • RN-tre
  • TRE2NL
  • USP6 N-terminal like
  • USP6 N-terminal-like protein

Background

Acts as a GTPase-activating protein for RAB5A. Involved in receptor trafficking. In complex with EPS8 inhibits internalization of EGFR.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for USP6NL Antibody (NBP2-20839) (0)

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Review for USP6NL Antibody (NBP2-20839) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-20839:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot USP6NL NBP2-20839
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reviewed by:
Anonymous
WB Human 12/15/2017
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Summary

ApplicationWestern Blot
Sample Testedliver and primary hepatocytes
SpeciesHuman
Lot40499

Comments

Commentshuman liver and primary hepatocytes
Name: Anti-USP6NL antibody (NBP2-20839)
Catalog #: Anti-USP6NL antibody (NBP2-20839)
Lot Number: Anti-USP6NL antibody (NBP2-20839, Lot # 40499)
PO/Order Number: Click here to enter text..
WB Image Description (Please provide labels for all lanes): lane 1: human liver; lane 2: human primary hepatocytes
Sample Information:
     Cell Line or Tissue: human liver, and human primary hepatocytes
     Species: human
     Treatment: No treatment
Lysate Preparation:
     Date of lysate preparation: December 15, 2017
     Lysis buffer used: 1X lysis buffer from Cell Signaling by adding PMSF
Reducing agent: beta-mercaptoethanol, DTT
If boiled (temperature/time): Yes
Controls:
     Positive Control: No
     Negative Control: No
     Loading Control (please attach additional images if applicable): No
Protein Amount Loaded per lane: 20 ug
Antibody Storage Conditions: -20℃
Electrophoresis:
Gel Percentage: 15%
Electrophoresis Conditions: Tris-Glycine-SDS at room temperature
     Voltage: 120V
     Time: 2 hours
Membrane Transfer:
     Method (Submersion/Semi-dry): wet transfer
     Membrane Type (PVDF/Nitrocellulose): Nitrocellulose
     Time: 2 hours
     Voltage: 100V
Blocking:
     Blocking Solution: 5% milk in 1X TBST
     Time: 1 hour at room temperature
Primary Antibody:
     Dilution: 1/5000
     Diluent Buffer: 2.5% BSA
     Incubation Time: overnight
     Incubation Temperature: 4℃
Washing Conditions:
     Wash Solution: 1X TBST
     Time and Repetitions: 5 min each for 3 times
Secondary Antibody
Manufacturer and Catalog #: Promega, W401B, Lot # 0000187662
Secondary description: goat anti-rabbit secondary antibody
     Dilution: 1/2000
     Diluent Buffer: 3% milk
     Incubation Time: 1 hour
     Incubation Temperature: room temperature
Detection Method:
Detection: ECL (GE, cat # RPN2209, lot # 9838243)
Procedure: Add equal volume of A and B, mix and apply on the membranes for 3-5 min before exposure
Development Time: 20 seconds
Molecular weight of band(s): ~ 97 kDa
Experimental Concerns and Observations: A specific band around 97 kDa was observed

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FAQs for USP6NL Antibody (NBP2-20839) (0)

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Secondary Antibodies

 

Isotype Controls

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Recent Reviews

4
5
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4
1
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2
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1
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Anonymous
12/15/2017
Application: WB
Species: Human

Bioinformatics

Gene Symbol USP6NL
Uniprot