Eps8 Antibody (18F1F2)

Western Blot: EPS8 Antibody (18F1F2) [NBP2-27300] - analysis of EPS8 partial recombinant protein using EPS8 antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: EPS8 Antibody (18F1F2) [NBP2-27300] - Formalin-fixed, paraffin-embedded human colon cancer stained with EPS8 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu
• Applications: WB, IHC, IHC-P
• Clone: 18F1F2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Eps8 Antibody (18F1F2) Summary

• Immunogen: Amino acids 701-882 of human EPS8 protein were used as the immunogen for the EPS8 antibody.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EPS8
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-7ug/ml~
  • Western Blot 1-5ug/ml
• Application Notes: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reactivity Notes

Human

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for Eps8 Antibody (18F1F2)

• epidermal growth factor receptor kinase substrate 8
• epidermal growth factor receptor pathway substrate 8
• Eps8

Background

EPS8, an acronym for epidermal growth factor receptor pathways substrate 8, is a signaling protein and part of the molecular complex controlling actin reorganization (1, 2). EPS8 also plays an important role in innate immunity. Through LPS activation of TLR4 and the co adaptor MyD88 protein, macrophages induce enhanced EPS8 activity typified by phagocytosis- a key innate anti-microbial defense response (3). EPS8 colocalizes with TLR4 in LPS activated macrophage immunocomplexes (3). Interestingly, EPS8 also is over expressed in malignancies including breast cancers, pancreatic cancers, and colon cancers, as examples (2-4). Antibody to EPS8 provides a tool for characterizing its functional roles in motility, adhesion, signaling pathways such as non-canonical Wnt signaling and expression in normal and neoplastic cells (5).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EPS8
• Uniprot:
  • Q12929()