EPS15 Antibody [Alexa Fluor® 700]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 700

EPS15 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human Eps15
  Met492-Thr579
  Accession # P42566
• Specificity: Detects human Eps15 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EPS15 Antibody [Alexa Fluor® 700]

• AF1P
• AF-1P
• ALL1 fused gene from chromosome 1
• epidermal growth factor receptor pathway substrate 15
• epidermal growth factor receptor substrate 15
• Eps15
• MLLT5
• Protein AF-1p
• Protein Eps15

Background

EPS15 (Epidermal growth factor receptor substrate 15) is a 138-140 kDa member of the EPS15 family of proteins. Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionary conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet aspartate-prolinephenylalanine that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin. Eps15 binds to AP-2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles.The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and thus implicated in different facets of endosomal trafficking. Human EPS15 has been mapped to chromosome 1p31- p32, a region displaying several non-random chromosomal abnormalities, including deletions in neuroblastoma and translocation in acute lymphoblastic and myeloid leukemias. Over aa 492-579, human EPS15 shares 76% aa identity with mouse EPS15.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.