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1195-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human Epiregulin is neutralized (green line) by increasing concentrations of Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1195). The ND50 is typically ≤1.5 µg/mL." class="big_lightbox" target="_blank">
MAB14252) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1195) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994)." class="big_lightbox" target="_blank">
1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068). The ND50 is typically 0.2-1 µg/mL." class="big_lightbox" target="_blank">
1068-EP) was serially diluted and captured by Rat Anti-Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF019). A specific band was detected for Proepiregulin at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox" target="_blank">
1068-EP) stimulates proliferation in the Balb/3T3 mouse embryonic fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Epiregulin (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068). The ND50 is typically 0.3-1 µg/mL." class="big_lightbox" target="_blank">
1068-EP) was serially diluted and captured by Rat Anti-Mouse Epiregulin Monoclonal Antibody (Catalog # MAB1068) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Epiregulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1068) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">