Ephrin-B [p Tyr317] Antibody [Unconjugated]

Images

 
Western blot of rat testis lysate showing specific immunolabeling of the approximately 45 kDa Ephrin-B phosphorylated at Y316 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Ch, XpSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Ephrin-B [p Tyr317] Antibody [Unconjugated] Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Y298 of Xenopus Ephrin-B
Modification
p Tyr317
Specificity
This antibody is specific for the 45 kDa Ephrin-B phosphorylated at Y316 in Western blots of rat testis.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EFNB1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000 dilution
Publications
Read Publication using
PPS047 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
For long-term storage, ≤ ‑20° C is recommended. Product is stable at ≤ ‑20° C for at least 1 year.
Buffer
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.
Preservative
No Preservative

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-B [p Tyr317] Antibody [Unconjugated]

  • craniofrontonasal syndrome (craniofrontonasal dysplasia)
  • EFL3
  • EFL-3
  • ELK ligand
  • Elk-L
  • EPH-related receptor tyrosine kinase ligand 2
  • EphrinB
  • Ephrin-B
  • ephrin-B1
  • EPLG2CFNS
  • LERK-2
  • LERK2CFND
  • ligand of eph-related kinase 2
  • MGC8782

Background

Ephrin-B1 (from ephoros; Greek for controller) is a 45 kDa, type I transmembrane glycoprotein found on a number of highly divergent cell types. It is a ligand for the Eph family of receptor tyrosine kinases, principally EphB1, 2 and 3. It participates in angiogenesis, T cell development and activation, platelet adhesion, neural crest migration and axonal patterning. In rodent, Ephrin-B1 contains a 212 amino acid (aa) extracellular domain (ECD) and an 88 aa cytoplasmic region. The ECD exhibits a characteristic four Cystein pattern, while the cytoplasmic region displays a three aa C‑terminal PZD binding motif (Tyr-Lys-Val). Upon receptor ligation, Ephrin-B1 is known to be phosphorylated on tyrosine residues. Based on motifs that are seven amino acids in length, three tyrosines are conserved, Xenopus to chicken to human to rodent (mouse; rat). The first tyrosine is Xenopus Y298, chicken Y305, human Y317, and rodent Y316. This is contained in a common CPHYEKV motif. The second tyrosine is Xenopus Y310, chicken Y317, human Y329, and rodent Y328. This is contained in a common HPVYIVQ motif. The third tyrosine is Xenopus Y324, chicken Y331, human Y343, and rodent Y343 that is contained in a common ANIYYKV motif. Phosphorylation on rodent Y316 results in SH2-containing Grb4 interaction. Phosphorylation of rodent Y328 and Y343 may contribute to receptor oligomerization and/or interaction with trimeric G‑proteins. Based on Ephrin-B2 studies, rodent Y328 may also participate in SH2-domain binding.
  1. Flanagan, J.C. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  2. Heroult, M. et al. (2006) Exp. Cell Res. 312:642.
  3. Kalo, M.S. et al. (2001) J. Biol. Chem. 276:38940.
  4. Bong, Y-S. et al. (2004) Biochem. J. 377:499.
  5. Song, J. (2003) J. Biol. Chem. 278:24714.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

AF638
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
AF467
Species: Hu, Mu
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, ICC
AF395
Species: Hu
Applications: WB, IHC
AF432
Species: Mu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-30646
Species: Hu
Applications: WB, IHC, IHC-P
8415-A2
Species: Mu
AF641
Species: Mu
Applications: WB, IHC
374-EA
Species: Hu
AF446
Species: Mu
Applications: WB, Flow, IHC, CyTOF-ready
MAB3035
Species: Hu
Applications: Flow, CyTOF-ready, ICC, KO
AF369
Species: Hu
Applications: WB, IHC, ICC
NBP2-20486
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-37364
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
359-EA
Species: Hu
NB100-56603
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P

Publications for Ephrin-B Antibody (PPS047)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


Filter By Application
IHC
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Reviews for Ephrin-B Antibody (PPS047) (0)

There are no reviews for Ephrin-B Antibody (PPS047). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Ephrin-B Antibody (PPS047) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Ephrin-B Products

Array PPS047

Bioinformatics Tool for Ephrin-B Antibody (PPS047)

Discover related pathways, diseases and genes to Ephrin-B Antibody (PPS047). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Ephrin-B Antibody (PPS047)

Discover more about diseases related to Ephrin-B Antibody (PPS047).
 

Pathways for Ephrin-B Antibody (PPS047)

View related products by pathway.

PTMs for Ephrin-B Antibody (PPS047)

Learn more about PTMs related to Ephrin-B Antibody (PPS047).

Blogs on Ephrin-B

There are no specific blogs for Ephrin-B, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Ephrin-B [p Tyr317] Antibody [Unconjugated] and receive a gift card or discount.

Bioinformatics

Gene Symbol EFNB1