EpCAM/TROP1 Antibody

Western Blot: EpCAM/TROP1 Antibody [NBP2-27361] - Analysis of EpCAM using EpCAM antibody. Human HCT116 cell lysate in the 1) absence and 2) presence of immunizing peptide probed with 3 ug/ml of EpCAM antibody. I goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody [NBP2-27361] - Formalin-fixed, paraffin-embedded human colon stained with EpCAM antibody (1:200), peroxidase-conjugate and DAB chromogen.

• Reactivity: Hu, Pm
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

EpCAM/TROP1 Antibody Summary

• Immunogen: A portion of amino acids 130-180 of human EpCAM was used as the immunogen for this antibody.
• Localization: Extracellular
• Predicted Species: Primate (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EPCAM
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 3-5ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:400

Reactivity Notes

The amino acid sequence used as immunogen is homologous to: chimp and orangutan (94%), mouse (76%), cow (74%), pig (72%), rat (72%), canine (70%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein A purified

Alternate Names for EpCAM/TROP1 Antibody

• 17-1A
• 323/A3
• ACSTD1
• antigen identified by monoclonal AUA1
• CD326 antigen
• CD326
• Cell surface glycoprotein Trop-1
• chromosome 4, surface marker (35kD glycoprotein)
• DIAR5
• EGP
• EGP-2
• EGP314
• EGP40
• EpCAM
• epithelial cell adhesion molecule
• Epithelial cell surface antigen
• Epithelial glycoprotein 314
• Epithelial glycoprotein
• ESA
• GA733-2
• GA733-2EGP34
• gp40
• hEGP314
• HNPCC8
• KS 1/4 antigen
• KS1/4
• KSAHEA125
• M1S2
• M4S1
• M4S1Ly74
• Major gastrointestinal tumor-associated protein GA733-2
• MIC18MH99
• MOC31
• TACST-1
• TACSTD1
• TROP1
• TROP1CD326
• Tumor-associated calcium signal transducer 1CO-17A

Background

EpCAM (Epithelial cell adhesion molecule) is a glycosylated, 30- to 40-kDa type I transmembrane protein that serves important roles in cell adhesion, cell proliferation, differentiation, migration, cell cycle regulation and is implicated in cancer and stem cell signaling. In mammals, EpCAM is predominantly located in intercellular spaces where epithelial cells form very tight junctions. Expression of EpCAM is usually down-regulated as epithelial cells terminally differentiate. However, EpCAM levels often rise again during regeneration or neoplastic transformations. EpCAM is comprised of an extracellular domain with epidermal growth factor (EGF)- and thyroglobulin repeat-like domains, a single transmembrane domain, and a short 26-amino acid intracellular domain called EpICD. The cleavage of the EpCAM ectodomain, EpEx, by TACE the protease (Tumor necrosis factor a converting enzyme) and its shedding has been shown to release its intracellular domain (Ep-ICD) which then translocates to the nucleus resulting in activation of oncogenic signaling. The association of Ep-ICD with FHL2 and Wnt pathway components Beta-catenin and Lef-1 forms a nuclear complex that binds DNA at Lef-1 consensus sites and induces gene transcription, leading to increased cell proliferation (Ralhan R, etal 2010 and Gostner JM, etal 2011). EpCAM acts as a homophilic cell-cell adhesion molecule with positive effects on cell adhesiveness and negative effects on cell motility and metastasis. EpCAM and its partner E-cadherin, promotes both epithelial integrity and epithelial morphogenesis (Slanchev k, etal 2009).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for EpCAM/TROP1 Antibody (NBP2-27361). (Showing 1 - 1 of 1 FAQ).

1. I'm looking for EpCAM antibody for rat. Do you have any antibody which can use FACS?
   • We unfortunately do not have any EpCAM antibodies validated in FACS for detection of the rat protein. I am sorry for any inconvenience. I do have three products to EpCAM that can detect the rat protein. These products have been validated to detect the native rat protein, so they may work for FACS however they have not yet been tested in that particular application. If you would like to try them in your experiment you would qualify for our Innovator's Reward Program. This program was designed to help scientists with the cost of their antibodies in return for valuable information. If you decide to test an antibody in a previously untested species or application we will issue you a credit for the purchase price of the antibody in exchange for a review of your experiment using our product.

Bioinformatics

• Gene Symbol: EPCAM
• Uniprot:
  • O55159(Rat)