EPB41L1 Overexpression Lysate (Native)

Images

 
Western Blot: EPB41L1 Overexpression Lysate (Adult Normal) [NBL1-10287] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for EPB41L1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

EPB41L1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202596 and based on accession number NM_177996. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens erythrocyte membrane protein band 4.1-like 1 (EPB41L1), transcript variant 2, mRNA.
Gene
EPB41L1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
87.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for EPB41L1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EPB41L1 Overexpression Lysate (Native)

  • band 4.1-like protein 1
  • DKFZp686H17242
  • erythrocyte membrane protein band 4.1-like 1
  • KIAA0338Neuronal protein 4.1,4.1N
  • MGC11072
  • neuron-type nonerythroid protein 4.1

Background

Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The protein encoded by this gene is a neuronally-enriched protein that is structurally similar to EPB41. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them has been determined. [provided by RefSeq]. Transcript Variant: This variant (2) has a different 5' UTR and multiple coding region differences compared to variant 1. The resulting isoform (b) has a shorter N-terminus and lacks two in-frame segments compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for EPB41L1 Lysate (NBL1-10287) (0)

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Bioinformatics

Gene Symbol EPB41L1