EOMES Antibody (1219A) [Unconjugated]

Mouse splenocytes were stained with Rat Anti-Mouse NKp46/NCR1 APC‑conjugated Monoclonal Antibody (Catalog # FAB22252A) and either (A) Rabbit Anti-Mouse EOMES Monoclonal Antibody (Catalog # MAB8889) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Phycoerythrin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0110).

• Reactivity: Mu
• Applications: Flow
• Clone: 1219A
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

EOMES Antibody (1219A) [Unconjugated] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: E. coli-derived recombinant mouse EOMES
  Met1-Ser126
  Accession # O54839
• Specificity: Detects mouse EOMES in direct ELISAs.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: EOMES
• Purity Statement: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: Sodium Azide
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EOMES Antibody (1219A) [Unconjugated]

• EOMES
• eomesodermin (Xenopus laevis) homolog
• Eomesodermin
• t box, brain, 2
• T-box brain protein 2
• T-box brain2
• TBR2
• TBR-2
• TBR2eomesodermin homolog
• T-brain-2

Background

EOMES (Eomesodermin), also TBR2, is a 72 kDa member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-gamma and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage. Human EOMES is 686 amino acids (aa) in length. It contains short poly-Ala, -Gly and -Asn motifs, and a DNA-binding T box (aa 276-456). There is one isoform that shows a 19 aa insertion after Ser460. Over aa 471‑686, human EOMES shares 91% aa identity with mouse EOMES.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EOMES Antibody (MAB8889)(5)

Publications using MAB8889

• Biniaris-Georgallis, SI;Aschman, T;Stergioula, K;Schreiber, F;Jafari, V;Taranko, A;Karmalkar, T;Kasapi, A;Lenac Rovis, T;Jelencic, V;Bejarano, DA;Fabry, L;Papacharalampous, M;Mattiola, I;Molgora, M;Hou, J;Hublitz, KW;Heinrich, F;Guerra, GM;Durek, P;Patone, G;Lindberg, EL;Maatz, H;Hölsken, O;Krönke, G;Mortha, A;Voll, RE;Clarke, AJ;Hauser, AE;Colonna, M;Thurley, K;Schlitzer, A;Schneider, C;Stamatiades, EG;Mashreghi, MF;Jonjic, S;Hübner, N;Diefenbach, A;Kanda, M;Triantafyllopoulou, A; Amplification of autoimmune organ damage by NKp46-activated ILC1 Nature 2024-08-13 [PMID: 39137897] (Immunohistochemistry, Mouse, Transgenic Mouse)
• Penny A. F. Whiley, Benedict Nathaniel, Peter G. Stanton, Robin M. Hobbs, Kate L. Loveland Spermatogonial fate in mice with increased activin A bioactivity and testicular somatic cell tumours Frontiers in Cell and Developmental Biology 2023-07-26 [PMID: 37564373]
• H Wang, L Shen, X Sun, F Liu, W Feng, C Jiang, X Chu, X Ye, C Jiang, Y Wang, P Zhang, M Zang, D Zhu, Y Bi Adipose group 1 innate lymphoid cells promote adipose tissue fibrosis and diabetes in obesity Nat Commun, 2019-07-22;10(1):3254. 2019-07-22 [PMID: 31332184] (Flow Cytometry, Human)
• C Kudo-Saito, A Ishida, Y Shouya, K Teramoto, T Igarashi, R Kon, K Saito, C Awada, Y Ogiwara, M Toyoura Blocking the FSTL1-DIP2A Axis Improves Anti-tumor Immunity Cell Rep, 2018-08-14;24(7):1790-1801. 2018-08-14 [PMID: 30110636] (Flow Cytometry, Mouse)
• HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs Identification of dynamic undifferentiated cell states within the male germline Nat Commun, 2018-07-19;9(1):2819. 2018-07-19 [PMID: 30026551] (IHC, Mouse)

FAQs for EOMES Antibody (MAB8889). (Showing 1 - 1 of 1 FAQs).

1. Please supply a list of eomes antibodies
   • Here is a link to all of our EOMES antibodies

Bioinformatics

• Gene Symbol: EOMES
• Uniprot:
  • O54839()