ENOX2 Antibody - Azide and BSA Free

Western Blot: ENOX2 Antibody [NBP3-05024] - Western blot analysis of extracts of Mouse lung, using ENOX2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic KitExposure time: 1s.

Western Blot: ENOX2 Antibody [NBP3-05024] - Western blot analysis of extracts of Rat lung, using ENOX2 antibody at 1:1000 dilutionSecondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilutionLysates/proteins: 25ug per laneBlocking buffer: 3% nonfat dry milk in TBSTDetection: ECL Basic KitExposure time: 1s

Immunocytochemistry/Immunofluorescence: ENOX2 Antibody [NBP3-05024] - Analysis of C6 cells using ENOx2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ENOX2 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit ENOX2 Antibody - Azide and BSA Free (NBP3-05024) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 411-610 of human ENOX2 (NP_872114.1). AEALKEENDSLRWQLDAYRNEVELLKQEQGKVHREDDPNKEQQLKLLQQALQGMQQHLLKVQEEYKKKEAELEKLKDDKLQVEKMLENLKEKESCASRLCASNQDSEYPLEKTMNSSPIKSEREALLVGIISTFLHVHPFGASIEYICSYLHRLDNKICTSDVECLMGRLQHTFKQEMTGVGASLEKRWKFCGFEGLKLT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENOX2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for ENOX2 Antibody - Azide and BSA Free

• COVA1
• Cytosolic ovarian carcinoma antigen 1APK1 antigen
• ecto-NOX disulfide-thiol exchanger 2
• tNOXAPK1
• Tumor-associated hydroquinone oxidase

Background

The protein encoded by the ENOX2 gene is a growth-related cell surface protein. It was identifed because it reacts with the monoclonal antibody KI in cells, such as the ovarian carcinoma line OVCAR-3, also expressing the CAKI surface glycoprotein. The encoded protein has two enzymatic activities: catalysis of hydroquinone or NADH oxidation, and protein disulfide interchange. The two activities alternate with a period length of about 24 minutes. The encoded protein also displays prion-like properties. Two transcript variants encoding different isoforms have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENOX2