eNOS [p Ser1177] Antibody (1014F) [PerCP]

• Reactivity: Hu
• Applications: WB
• Clone: 1014F
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: PerCP

eNOS [p Ser1177] Antibody (1014F) [PerCP] Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Phosphopeptide containing the human eNOS S1177 site
  Accession # P29474
• Modification: p Ser1177
• Specificity: Detects human eNOS when phosphorylated at S1177 in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NOS3
• Purity: Protein A or G purified from cell culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from cell culture supernatant

Alternate Names for eNOS [p Ser1177] Antibody (1014F) [PerCP]

• Constitutive NOS
• EC 1.14.13.39
• ECNOS
• Endothelial NOS
• endothelial
• eNOS
• eNOScNOS
• nitric oxide synthase 3 (endothelial cell)
• NOS type III
• NOS3
• NOSIII

Background

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. iNOS is found in a variety of cell types including macrophages, hepatocytes, synoviocytes, and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOS3