Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) - BSA Free

Immunohistochemistry: Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) [NBP3-30894] - Left and Center: Immunohistochemical staining of normal pancreas tissue (left) and small bowel tumor tissue (center) using NSE antibody (including NBP3-30894). Right: Western blot analysis using NSE antibodies (including NBP3-30894) on alpha and gamma enolase standards.

• Reactivity: Hu, Mu, Ch
• Applications: WB, ELISA, IHC
• Clone: NSE-P1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) - BSA Free Summary

• Immunogen: Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a synthetic peptide derived from the human Enolase 2/Neuron-specific Enolase protein and mouse myeloma Ag8563 cells. Sequence common in human, mouse and chicken.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin : 1-5 ug/ml
  • Western Blot : 1-2 ug/ml
• Application Notes: .

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Phosphate buffered saline
• Preservative: 0.08% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (NSE-P1) - BSA Free

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2