Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained with NSE gamma Monoclonal Antibody (ENO2/1462).

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Rat Heart stained with NSE gamma Monoclonal Antibody (ENO2/1462).

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained with NSE gamma Monoclonal Antibody (ENO2/1462)

Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free [NBP2-59603] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu, Mu, Rt
• Applications: IHC, MA, CyTOF-ready
• Clone: ENO2/1462
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-59602).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: A synthetic peptide of human Enolase 2/Neuron-specific Enolase (around aa416-433) (exact sequence is proprietary) (Uniprot: P09104)
• Localization: Cytoplasmic
• Marker: Neuroendocrine Marker
• Specificity: The specificity of this monoclonal antibody to its intended target was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Protein Array
• Application Notes: Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) - Azide and BSA Free

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2