Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462)

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Rat Heart stained withNSE gamma Monoclonal Antibody (ENO2/1462).

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Human Pheochromocytoma stained withNSE gamma Monoclonal Antibody (ENO2/1462).

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Formalin-fixed, paraffin-embedded Mouse Pancreas stained withNSE gamma Monoclonal Antibody (ENO2/1462)

SDS-Page: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602]

Protein Array: Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) [NBP2-59602] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, IHC-P, PA, PAGE
• Clone: ENO2/1462
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462) Summary

• Description: Human Chromosome Location:12p13
  Positive Control:HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
• Immunogen: A synthetic peptide of human NSE gamma (around aa416-433; exact sequence is proprietary)
• Localization: Cytoplasmic
• Marker: Neuroendocrine Marker
• Specificity: The specificity of this monoclonal antibody to human Enolase 2/Neuron-specific Enolase was validated by HuProtTM Array, containing more than 19,000, full-length human proteins. Recognizes a protein of about 50kDa, which is identified as gamma -enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5-1 ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.1-0.2 ug/ml
  • Protein Array 1:100-1:2000
  • SDS-Page
• Application Notes: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 -20 min followed by cooling at RT for 20 minutes
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (ENO2/1462)

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2