Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mouse mAb against Mouse brain (1), NIH3T3 (2), and C6 (3) cell lysate.

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of liver cancer tissues using ENO2 mouse mAb with DAB staining.

Flow Cytometry: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of HeLa cells using ENO2 mouse mAb (green) and negative control (red).

Western Blot: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis using ENO2 mAb against human ENO2 recombinant protein. (Expected MW is 46 kDa)

Immunohistochemistry-Paraffin: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Analysis of lung cancer tissues using ENO2 mouse mAb with DAB staining.

ELISA: Enolase 2/Neuron-specific Enolase Antibody (5D3) [NBP2-37465] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

• Reactivity: Hu, Mu
• Applications: WB, ELISA, Flow, IHC
• Clone: 5D3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human Enolase 2/Neuron-specific Enolase (AA: 251-433) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ENO2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 47.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-questions.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Enolase 2/Neuron-specific Enolase Antibody (5D3) - BSA Free

• 2-phospho-D-glycerate hydrolyase
• 2-phospho-D-glycerate hydro-lyase
• EC 4.2.1.11
• ENO2
• enolase 2 (gamma, neuronal)
• Enolase 2
• gamma-Enolase
• Neural enolase
• neuron specific gamma enolase
• neurone-specific enolase
• Neuronspecific Enolase
• Neuron-specific enolase
• NSE

Background

Neuron-specific enolase (NSE) is a glycolytic isoenzyme which is located in central and peripheral neurons and neuroendocrine cells. This enzyme is released into the CSF when neural tissue is injured. Neoplasms derived from neural or neuroendocrine tissue may release NSE into the blood. NSE is a useful substance that has been detected in patients with certain tumors, namely: neuroblastoma, small cell lung cancer, medullary thyroid cancer, carcinoid tumors, pancreatic endocrine tumors, and melanoma. Recombinant NSE was expressed in E.coli and purified by conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ENO2