Endothelial Lipase Antibody

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Western Blot: Endothelial Lipase Antibody [NBP1-05031] - Analysis of Endothelial Lipase in Human Liver lysate using NBP1-05031 at 0.3ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Endothelial Lipase Antibody Summary

Immunogen
Peptide with sequence C-RFNLRTSKDPEHEG corresponding to internal region according to NP_006024.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIPG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 0.3 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 70 kDa band observed in human liver lysates is reported in scientific literature (PMID: 17356047). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Positive Control
Endothelial Lipase Lysate (NBL1-12550)
Publications
Read Publications using NBP1-05031.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Endothelial Lipase Antibody

  • EC 3.1.1
  • EC 3.1.1.3
  • EDLendothelial lipase
  • ELlipoprotein lipase H
  • Endothelial cell-derived lipase
  • lipase, endothelial
  • PRO719

Background

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endothelial Lipase Antibody (NBP1-05031)(2)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIPG
Entrez
Uniprot