Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

Western Blot: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Endothelial Lipase (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-End

Immunocytochemistry/Immunofluorescence: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Endothelial Lipase.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Flow Cytometry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - HEK293T cells transfected with either Endothelial Lipase Human Tagged ORF Clone overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-LIPG antibody , and then analyzed by flow cytometry.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Carcinoma of Human liver tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Human breast tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Immunohistochemistry: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Staining of paraffin-embedded Human Kidney tissue using anti-Endothelial Lipase mouse monoclonal antibody.

Immunoprecipitation: Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free [NBP2-71309] - Lane 1: Negative Control Lane 2: NBP2-02378 Lane 3: NBP2-02380 Lane 4:NBP2-02381 Lane 5: NBP2-02382 Lane 6: NBP2-02394 Lane 7: NBP2-02395 Lane 8: NBP2-02396 Lane 9: NBP2-02397

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: OTI1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free (NBP2-02396) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human LIPG (NP_006024) produced in HEK293T cell.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: LIPG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:2000
• Theoretical MW: 54.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Endothelial Lipase Antibody (OTI1D9) - Azide and BSA Free

• EC 3.1.1
• EC 3.1.1.3
• EDLendothelial lipase
• ELlipoprotein lipase H
• Endothelial cell-derived lipase
• lipase, endothelial
• PRO719

Background

Endothelial lipase (EL) is a novel member of the lipoprotein lipase gene family. Endothelial lipase is synthesized by endothelial cells and functions at the site of synthesis. EL most likely plays a physiological role in modulating lipoprotein metabolism. Overexpressed EL has been shown to reduce HDL cholesterol levels, whereas an Endothelial lipase deficiency appears to be effective in elevating HDL cholesterol levels in animals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LIPG