Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) [CoraFluor™ 1]

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF, IHC, IP, AP
• Clone: A76
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: CoraFluor 1

Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) [CoraFluor™ 1] Summary

• Description: CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
• Immunogen: Lysed bovine corneal endothelial cells and extracellular matrix
• Specificity: Highly specific for perlecan. There is no evidence for cross-reactivity with other connective tissue proteins (vitronectin, fibronectin, elastin, collagen, laminin).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HSPG2
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark. Do not freeze.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Endorepellin/Perlecan/Heparan Sulfate Proteoglycan Antibody (A76) [CoraFluor™ 1]

• basement membrane-specific heparan sulfate proteoglycan core protein
• endorepellin (domain V region)
• heparan sulfate proteoglycan 2
• Heparan Sulfate Proteoglycan
• HSPG
• perlecan proteoglycan
• Perlecan
• PLCSchwartz-Jampel syndrome 1 (chondrodystrophic myotonia)
• PRCAN
• SJA
• SJS
• SJS1

Background

Perlecan is an extracellular matrix proteoglycan. It has a large core protein of 400-450 kDa and is often produced with heparan sulfate side chains. Perlecan is found in basement membranes where it contributes to the permeability characteristics, serves as a substrate for vascular cells and binds growth factors involved in vascular remodeling (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HSPG2