Endoglycan/PODXL2 Antibody [Unconjugated]

Endoglycan/PODXL2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Endoglycan/PODXL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Endoglycan/PODXL2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
  Gly33-Thr500
  Accession # Q9NZ53
• Specificity: Detects human Endoglycan/PODXL2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human PODXL.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: PODXL2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglycan/PODXL2 Antibody [Unconjugated]

• Endoglycan
• PODLX2
• podocalyxin-like 2
• podocalyxin-like protein 2
• PODXL2

Background

Endoglycan, also named podocalyxin-like 2 (PODXL2), is a member of the CD34 family of sialomucins. It is a Type I transmembrane protein expressed on endothelial cells, early hematopoietic progenitors and leukocyte subpopulations. Specific endoglycan glycoforms function as L-Selectin ligands.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglycan/PODXL2 Antibody (AF1524)(1)

Publication using AF1524

• Kerr SC, Fieger CB, Snapp KR, Rosen SD Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins. J. Immunol., 2008-07-15;181(2):1480-90. 2008-07-15 [PMID: 18606703] (Flow Cytometry, Western Blot, Human)

Bioinformatics

• Gene Symbol: PODXL2
• Uniprot:
  • Q9NZ53()