Endoglycan/PODXL2 Antibody (211816) [Unconjugated]

Endoglycan/PODXL2 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human Endoglycan/PODXL2 Monoclonal Antibody (Catalog # MAB1524) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready, ICC/IF
• Clone: 211816
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Endoglycan/PODXL2 Antibody (211816) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Endoglycan/PODXL2
  Gly33-Thr500
  Accession # Q9NZ53
• Specificity: Detects human Endoglycan/PODXL2 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL2
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Endoglycan/PODXL2 Antibody (211816) [Unconjugated]

• Endoglycan
• PODLX2
• podocalyxin-like 2
• podocalyxin-like protein 2
• PODXL2

Background

Endoglycan, also named Podocalyxin-like 2 protein, is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. It is expressed in hematopoietic precursors, endothelial cells, smooth muscle cells and leukocyte subpopulations. Endoglycan is a potential marker for hematopoietic progenitors. It also functions as an L-Selectin ligand and may be involved in the inhibition of stem cell differentiation and leukocyte-endothelial adhesion (1, 2).

1. Sassetti, C. et al. (2000) J. Biol. Chem. 275:9001.
2. Fieger, C.B. et al. (2003) J. Biol. Chem. 278:27390.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglycan/PODXL2 Antibody (MAB1524)(2)

Publications using MAB1524

• D Dabbagh, S He, B Hetrick, L Chilin, A Andalibi, Y Wu Identification of the SHREK family of proteins as broad-spectrum host antiviral factors bioRxiv : the preprint server for biology, 2021-02-03;0(0):. 2021-02-03 [PMID: 33564770] (ELISA Capture, ICC, Human)
• Kerr SC, Fieger CB, Snapp KR, Rosen SD Endoglycan, a member of the CD34 family of sialomucins, is a ligand for the vascular selectins. J. Immunol., 2008-07-15;181(2):1480-90. 2008-07-15 [PMID: 18606703] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: PODXL2
• Uniprot:
  • Q9NZ53()