Endo G Antibody

Product Discontinued

Endo G Antibody Summary

• Immunogen: Synthetic peptide: GGPRGPGELAKYGLP, corresponding to amino acids 55-70 of Human Endonuclease G.
• Localization: Mitochondrial
• Specificity: Endonuclease G
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ENDOG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10-15 ug/ml
  • Western Blot 1-2 ug/ml
• Application Notes: Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa), determined using mouse 3T3 (fibroblasts) and human HepG2 (hepatoblastoma) cell lysates.
• Theoretical MW: 35 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Cross-reacts with Human, Mouse and Rat. Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Endo G Antibody

• EC 3.1.30.-
• Endo G
• endonuclease G
• endonuclease G, mitochondrial
• FLJ27463
• mitochondrial endonuclease G

Background

The fragmentation of nuclear DNA is a hallmark of apoptotic cell death. The activities of caspase and nuclease are involved in the DNA fragmentation. Caspase-activated deoxyribonuclease (CAD), also termed DNA fragmentation factor (DFF40), is one such nuclease, and is capable of inducing DNA fragmentation and chromatin condensation after cleavage by caspase-3 of its inhibitor ICAD/DFF45. Caspase and CAD independent DNA fragmentation also exists. Recent studies demonstrated that another nuclease, endonuclease G (endoG), is specifically activated by apoptotic stimuli and is able to induce nucleosomal fragmentation of DNA independently of caspase and DFF/CAD (1,2). EndoG is a mitochondrion-specific nuclease that translocates to the nucleus and cleaves chromatin DNA during apoptosis. The homologue of mammalian EndoG is the first mitochondrial protein identified to be involved in apoptosis in C. elegans (2). EndooG also cleaves DNA in vitro (4).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endo G Antibody (NB120-10522)(2)

Publications using NB120-10522

• Jacquemin G, Margiotta D, Kasahara A et al. Granzyme B-induced mitochondrial ROS are required for apoptosis Cell Death Differ. 2014-10-31 [PMID: 25361078] (WB, Mouse, Human)
  
  Details:
  Endo G antibody used for WB on supernatants and mitochondrial pellets from purified intact mouse liver mitochondria treated with Granzyme B in the presence or absence of S100 cytosolic fraction (Figure 3). Antibody also used on total cell lysate, cytosolic and mitochondrial fractions of U937 cells treated or not with perforin and Granzyme B, with or without n-acetyl cysteine/nAC pretreatment (Figure 4).
• Srivastava V, Modi P, Tripathi V et al. BLM helicase stimulates the ATPase and chromatin-remodeling activities of RAD54. J Cell Sci 2009-09-01 [PMID: 19671661]

Bioinformatics

• Gene Symbol: ENDOG
• Entrez:
  • 2021(Human)
  • 362100(Rat)