EMP/MAEA Overexpression Lysate


Western Blot: MAEA Overexpression Lysate (Adult Normal) [NBL1-12791] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MAEA.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

EMP/MAEA Overexpression Lysate Summary

Transient overexpression lysate of macrophage erythroblast attacher (MAEA), transcript variant 1 The lysate was created in HEK293T cells, using Plasmid ID RC200147 and based on accession number NM_001017405. The protein contains a C-MYC/DDK Tag.


Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
45.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for Array



HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for EMP/MAEA Overexpression Lysate

  • EMLP
  • EMP
  • EMPCell proliferation-inducing gene 5 protein
  • Erythroblast macrophage protein
  • HLC-10
  • Human lung cancer oncogene 10 protein
  • lung cancer-related protein 10
  • macrophage erythroblast attacher
  • MAEA
  • PIG5
  • proliferation-inducing gene 5


This gene product mediates the attachment of erythroblasts to macrophages. This attachment promotes terminal maturation and enucleation of erythroblasts, presumably by suppressing apoptosis. This protein is an integral membrane protein with the N-terminus on the extracellular side and the C-terminus on the cytoplasmic side of the cell. Two immunologically related isoforms of erythroblast macrophage protein with apparent molecular weights of 33 kD and 36 kD were detected in macrophage membranes; this gene encodes the larger isoform. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the biological validity of some variants has not been determined. Transcript Variant: This variant (1) is the longer transcript and encodes the longer isoform (1).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol MAEA