EMMPRIN/CD147 Antibody [Unconjugated]

Western blot shows lysates of mouse brain tissue, mouse heart tissue, and mouse small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMMPRIN/CD147 at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of mouse brain (cortex) tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for EMMPRIN/CD147 at approximately 62 kDa (as indicated) using 2 µg/mL of Goat Anti-Mouse EMMPRIN/CD147 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF772) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, Flow, CyTOF-ready
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EMMPRIN/CD147 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
  Ala22-Arg209
  Accession # NP_001070652
• Specificity: Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human EMMPRIN is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: BSG
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • CyTOF-ready

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMMPRIN/CD147 Antibody [Unconjugated]

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMMPRIN/CD147 Antibody (AF772)(4)

Publications using AF772

• Kato N, Kosugi T, Sato W, Ishimoto T, Kojima H, Sato Y, Sakamoto K, Maruyama S, Yuzawa Y, Matsuo S, Kadomatsu K Basigin/CD147 promotes renal fibrosis after unilateral ureteral obstruction. Am. J. Pathol., 2011;178(2):572-9. 2011 [PMID: 21281789] (Western Blot, Mouse)
• Kato N, Yuzawa Y, Kosugi T The E-selectin ligand basigin/CD147 is responsible for neutrophil recruitment in renal ischemia/reperfusion. J. Am. Soc. Nephrol., 2009;20(7):1565-76. 2009 [PMID: 19443639] (Western Blot, Mouse)
• Chen L, Belton RJ, Nowak RA Basigin-mediated gene expression changes in mouse uterine stromal cells during implantation. Endocrinology, 2009;150(2):966-76. 2009 [PMID: 18832103] (IHC-P, Mouse)
• Ramo K, Cashman SM, Kumar-Singh R Evaluation of adenovirus-delivered human CD59 as a potential therapy for AMD in a model of human membrane attack complex formation on murine RPE. Invest. Ophthalmol. Vis. Sci., 2008;49(9):4126-36. 2008 [PMID: 18487376] (ICC, Mouse)

Bioinformatics

• Gene Symbol: BSG
• Uniprot:
  • NP_001070652()