EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) purified, GAM-APC.

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) biotin.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Surface staining of human peripheral blood with anti-human CD147 (MEM-M6/1) FITC.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) Alexa Fluor® 488.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) PE.

Flow Cytometry: EMMPRIN/CD147 Antibody (MEM-M6/1) [NB500-430] - Staining of human peripheral blood with anti-human CD147 (MEM-M6/1) APC.

• Reactivity: Hu, Eq
• Applications: WB, ELISA, Flow, IHC, IP, CyTOF-ready
• Clone: MEM-M6/1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free Summary

• Immunogen: Protein A-CR purified soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the hinge region, C2-and C3 domain of human IgG1, CD147Rg is secreted by transfectants as a dimer (NM_001728.2).
• Specificity: The antibody MEM-M6/1 recognizes an epitope in the N-terminal Ig domain (D1) of CD147 (Neurothelin), a 50-60 kDa type I transmembrane glycoprotein primarily expressed on all leukocytes, red blood cells, platelets and endothelial cells; it is not expressed by resting lymphocytes. The antibody MEM-M6/1 is a high-affinity antibody capable of binding to unstimulated peripheral blood T cells.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100-1:2000
  • Flow Cytometry 10 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation 1:50
  • Western Blot 1 ug/ml
• Application Notes: Western Blot - Non-reducing conditions. Use in ELISA reported in scientific literature (PMID 24710016). This antibody is CyTOF ready.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  EMMPRIN/CD147 Overexpression Lysate

Reactivity Notes

Equine reactivity reported in scientific literature (PMID: 24998980).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for EMMPRIN/CD147 Antibody (MEM-M6/1) - BSA Free

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EMMPRIN/CD147 Antibody (NB500-430)(4)

Publications using NB500-430

• Bortolotti D, Simioni C, Neri LM Et al. Relevance of VEGF and CD147 in different SARS-CoV-2 positive digestive tracts characterized by thrombotic damage FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2021-12-01 [PMID: 34822202] (IF/IHC, Human)
• Joeh E, O'Leary T, Li W et al. Mapping glycan-mediated galectin-3 interactions by live cell proximity labeling Proc Natl Acad Sci U S A 2020-10-17 [PMID: 33067390]
• Uhl PB, Szober CM, Amann B et al. In situ cell surface proteomics reveals differentially expressed membrane proteins in retinal pigment epithelial cells during autoimmune uveitis. J Proteomics 2014-07-02 [PMID: 24998980] (IHC-P, FLOW, Equine)
  
  Details:
  Basigin/CD147 antibody used at 1:10 dilution for FLOW application on healthy and uveitic equine RPE cells - harvested with Trypsin/EDTA (see full text for protocol details; data shown in Fig 1). Antibody also used for IHC-P on Bouin's solution fixed posterior eye cups-paraffin sections, EDTA-NaOH heat antigen retrieval, blocked with 1% BSA-TBST + 5% serum from host of secondary, primary used at 1:50 dilution ON 4C incubation, secondary used was anti-mouse IgG Alexa Fluor 647 (Fig 3, Fig 5).
• Yoshioka Y, Kosaka N, Konishi Y et al. Ultra-sensitive liquid biopsy of circulating extracellular vesicles using ExoScreen. Nat Commun 2014-04-08 [PMID: 24710016] (ELISA, WB, Human)

Bioinformatics

• Gene Symbol: BSG
• Entrez:
  • 682(Human)
• Uniprot:
  • P35613(Human)