EMMPRIN/CD147 Antibody (BSG/9223R) - Azide and BSA Free

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (BSG/9223R) - Azide and BSA Free [NBP3-24075] - Formalin-fixed, paraffin-embedded human placenta stained with EMMPRIN/CD147 Antibody (BSG/9223R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: ELISA, IHC
• Clone: BSG/9223R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

EMMPRIN/CD147 Antibody (BSG/9223R) - Azide and BSA Free Summary

• Description: Antibody without azide - store at -20 to -80C.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human EMMPRIN/CD147 protein
• Localization: Cell surface.
• Specificity: This monoclonal antibody recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: BSG
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry-Paraffin 1-2ug/ml
• Application Notes: ELISA: For coating, order antibody without BSA
  
  Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for EMMPRIN/CD147 Antibody (BSG/9223R) - Azide and BSA Free

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Background

CD147 plays a pivotal role in spermatogenesis, embryo implantation, neural network formation and tumor progression. It stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). CD147 may target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. CD147 also seems to be a receptor for oligomannosidic glycans. In vitro, it promotes outgrowth of astrocytic processes. CD147 forms homooligomers in a cis-dependent manner on the plasma membrane. It forms a complex with MMP1 at the tumor cell surface. CD147 interacts with SLC16A1 and SLC1A3; probably a BSG dimer is associated with a monocarboxylate transporter dimer. CD147 also interacts with ATP1B2, MAG and L1CAM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG