EMMPRIN/CD147 Antibody (8D6)

Immunohistochemistry-Paraffin: EMMPRIN/CD147 Antibody (8D6) [NBP2-33015] - Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD147 Monoclonal Antibody (8D6).

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
• Clone: 8D6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

EMMPRIN/CD147 Antibody (8D6) Summary

• Immunogen: Human T cell line Molt 13
• Localization: Cell surface
• Specificity: This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BSG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1:100-1:200
  • Immunohistochemistry -frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0ug/ml
  • Immunoprecipitation 0.5-1ug/500ug protein lysate
• Application Notes: Human Chromosome Location: 19p13.3
  Mol. Weight of Antigen: 35kDa (non-reduced); 40kDa (reduced)
  Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migation through Matrigel (Order Ab without BSA/Azide); Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Control:

  EMMPRIN/CD147 Knockout 293T Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein G purified

Alternate Names for EMMPRIN/CD147 Antibody (8D6)

• 5F7
• basigin (Ok blood group)
• Basigin
• BSG
• CD147 antigen
• CD147
• Collagenase stimulatory factor
• EMMPRIN
• EMMPRINTCSF
• Extracellular matrix metalloproteinase inducer
• Leukocyte activation antigen M6
• M6
• OK blood group antigen
• OK
• Tumor cell-derived collagenase stimulatory factor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BSG
• Uniprot:
  • P35613(Mouse)