EMG1 Antibody - Azide and BSA Free

Western Blot: EMG1 Antibody [H00010436-B01P] - Analysis of EMG1 expression in transfected 293T cell line by EMG1 polyclonal antibody. Lane 1: EMG1 transfected lysate(26.84 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: EMG1 Antibody [H00010436-B01P] - Analysis of purified antibody to EMG1 on HeLa cell. (antibody concentration 10 ug/ml)

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

EMG1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse EMG1 Antibody - Azide and BSA Free (H00010436-B01P) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: EMG1 (AAH55314, 1 a.a. - 244 a.a.) full-length human protein. MAAPSDGFKPRERSGGEQAQDWDALPPKRPRLGAGNKIGGRRLIVVLEGASLETVKVGKTYELLNCDKHKSILLKNGRDPGEARPDITHQSLLMLMDSPLNRAGLLQVYIHTQKNVLIEVNPQTRIPRTFDRFCGLMVQLLHKLSVRAADGPQKLLKVIKNPVSDHFPVGCMKVGTSFSIPVVSDVRELVPSSDPIVFVVGAFAHGKVSVEYTEKMVSISNYPLSAALTCAKLTTAFEEVWGVI
• Specificity: EMG1 - EMG1 nucleolar protein homolog (S. cerevisiae),
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: EMG1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500
• Application Notes: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. It has also been used for immunofluorescence.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EMG1 Antibody - Azide and BSA Free

• 18S rRNA (pseudouridine-N1-)-methyltransferase NEP1
• BWCNS
• C2F18S rRNA Psi1248 methyltransferase
• EMG1 nucleolar protein homolog (S. cerevisiae)
• essential for mitotic growth 1
• FLJ60792
• Grcc2f
• NEP1EC 2.1.1.-
• Nucleolar protein EMG1 homolog
• probable ribosome biogenesis protein NEP1
• Protein C2f
• Ribosome biogenesis protein NEP1

Background

EMG1 plays a role in the processing of the pre-18S rRNA and small ribosomal subunit assembly (Liu and Thiele, 2001 [PubMed 11694595]) and may play a role in methylation during ribosome biogenesis (Eschrich et al., 2002 [PubMed 11935223]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EMG1
• Entrez:
  • 10436(Human)
• Uniprot:
  • AAH55314(Human)