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WDR3 Antibody

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Western Blot: WDR3 Antibody [NB100-77296] - Detection of Human WDR3 on HeLa whole cell lysate using NB100-77296. WDR3 was also immunoprecipitated by another rabbit anti-WDR3 antibody.
Independent Antibodies: Western Blot: WDR3 Antibody [NB100-77296] - Detection of Human WDR3 on HeLa whole cell lysate using NB100-77296. WDR3 was also immunoprecipitated by another rabbit anti-WDR3 antibody.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml
Validated by:
 

Independent Antibodies

  

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WDR3 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 225 and 275 of human WD repeat-containing domain 3 using the numbering given in entry NP_006775.1 (GeneID 10885).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
WDR3
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:2000-1:10000
  • Immunoprecipitation 2-5 ug/mg lysate
Theoretical MW
106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NB100-77296.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for WDR3 Antibody

  • dJ776P7.2 (WD repeat domain 3)
  • FLJ12796
  • WD repeat domain 3
  • WD repeat-containing protein 3

Background

This gene encodes a nuclear protein containing 10 WD repeats. WD repeats are approximately 30- to 40-amino acid domains containing several conserved residues, which usually include a trp-asp at the C-terminal end. Proteins belonging to the WD repeat family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry: NCKIPSD Antibody [NBP1-42664] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SPIN90 used at a dilution of 1:500 (0.4ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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NCOA2 Antibody
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Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative images taken from estradiol-treated wt mice. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.
Immunohistochemistry: NCOA2 Antibody [NB100-1756] - Sample : FFPE section of prostate adenocarcinoma. Antibody : Affinity purified rabbit anti-NCOA2/SRC2 used at a dilution of 1:100. Detection : DAB
Immunocytochemistry/Immunofluorescence: NCOA2 Antibody [NB100-1756] - The majority of estradiol-induced PR-A or PR-B cells in the VMN of female mice coexpress SRC-1 and SRC-2. Representative image taken from vehicle control wt mice Insets show the magnified image of an area within the small square box. Magnification: images, 400x; insets, 630x. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (eneuro.sfn.org/cgi/doi/10.1523/ENEURO.0012-15.2015), licensed under a CC-BY licence.
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NB100-56599
Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.
Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.
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Applications: WB, Flow, ICC/IF, Flow-IC
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Western Blot: GRIP1 Antibody [NBP2-41211] - Analysis of GRIP1 in 293 cell lysate with GRIP1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
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Western Blot: EMG1 Antibody (OTI1B8) - Azide and BSA Free [NBP2-71601] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EMG1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EMG1.
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[Unconjugated]
291-G1
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NBP2-76849
Immunocytochemistry/Immunofluorescence: Mind Bomb 1/MIB1 Antibody (JG69-34) [NBP2-76849] - ICC staining Mib1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry: Mind Bomb 1/MIB1 Antibody (JG69-34) [NBP2-76849] - Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Mib1 antibody. Counter stained with hematoxylin.
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ELISA Kit (Colorimetr ...
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Western Blot: HN1L Antibody [NBP1-88446] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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Simple Western lane view shows lysates of human and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Galectin‑4 at approximately 44 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Galectin‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1227) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
(1 Publication)
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Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-19819] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.
(1 Review)
(1 Publication)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
UTP6 Antibody
NBP1-88468
Western Blot: UTP6 Antibody [NBP1-88468] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-UTP6 antibody. Remaining relative intensity is presented.
Immunocytochemistry/Immunofluorescence: UTP6 Antibody [NBP1-88468] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoli.
Immunohistochemistry-Paraffin: UTP6 Antibody [NBP1-88468] - Staining of human tonsil shows strong positivity in nucleoli in lymphoid cells.
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, KD
Glycophorin A
Antibody (R10) [Uncon ...
MAB1228
Glycophorin A was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes of red blood cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
TF‑1 human erythroleukemic cell line was stained with Mouse Anti-Human Glycophorin A Monoclonal Antibody (Catalog # MAB1228, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B).
(2 Publications)
Species: Hu
Applications: Flow, IHC, IP, CyTOF-ready

Publications for WDR3 Antibody (NB100-77296)(1)


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Bioinformatics

Gene Symbol WDR3
Entrez
Uniprot