EMAP-II/AIMP1 Antibody [Unconjugated]

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human EMAP-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for EMAP-II at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMAP-II was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Human EMAP-II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial and endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and OVCAR‑3 human ovarian carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for EMAP‑II at approximately 45 kDa (as indicated) using 10 µg/mL of Goat Anti-Human EMAP‑II Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1910) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EMAP-II/AIMP1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human EMAP-II
  Ser147-Lys312
  Accession # Q12904
• Specificity: Detects human and mouse EMAP-II in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AIMP1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EMAP-II/AIMP1 Antibody [Unconjugated]

• AIMP1
• aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• EMAP-2
• EMAPII
• EMAP-II
• EMAPIImember 1 (endothelialmonocyte-activating)
• endothelial-monocyte activating polypeptide II
• Multisynthase complex auxiliary component p43
• multisynthetase complex auxiliary component p43
• SCYE1

Background

EMAP-II is a pro-inflammatory cytokine that is chemotactic for monocytes and granulocytes. It is a cleavage product of multisynthetase complex auxiliary component p43.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AIMP1
• Uniprot:
  • Q12904()