ELYS Antibody

Immunocytochemistry/Immunofluorescence: ELYS Antibody [NBP1-87952] - Staining of human cell line A-431 shows localization to nuclear membrane. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human pancreas shows strong positivity in nuclear membrane of exocrine glandular cells.

Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human testis shows strong positivity in nuclear membrane of cells in seminiferous ducts.

Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human rectum shows strong positivity in nuclear membrane of glandular cells.

Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human prostate shows strong positivity in nuclear membrane of glandular cells.

Immunohistochemistry-Paraffin: ELYS Antibody [NBP1-87952] - Staining of human endometrium shows moderate positivity in nuclear membrane in glandular cells.

• Reactivity: Hu, Bv
• Applications: ChIP, ICC/IF, IHC, IHC-P, PLA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ELYS Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: GEEARNLSFNELYPSGTLKLQYNFDTIDQQFCDLADNKDTAECDIAEVDGELFVAQSNFTLILEGEEGEVEPGDFASSDVLPKAANTATEEKLVCSGEND
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AHCTF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Chromatin Immunoprecipitation
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Proximity Ligation Assay
• Application Notes: Use in Chromatin Immunoprecipitation reported in scientific literature (PMID:35017527) For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. Use in Proximity Ligation Assay reported in scientific literature (PMID:31784729).
• Control Peptide:

  ELYS Protein (NBP1-87952PEP)

Reactivity Notes

Use in Bovine reported in scientific literature (PMID: 33964210).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ELYS Antibody

• AT hook containing transcription factor 1
• ELYS transcription factor like protein TMBS62
• ELYS transcription factor-like protein TMBS62
• ELYS
• MST108
• MSTP108
• protein ELYS
• putative AT-hook-containing transcription factor 1
• TMBS62

Background

ELYS is a novel nuclear transcription factor expressed in embryonic haematopoietic tissues and plays a role in both early and mature haematopoietic events.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ELYS Antibody (NBP1-87952)(4)

Publications using NBP1-87952

• Scholz BA, Sumida N, de Lima CDM et al. WNT signaling and AHCTF1 promote oncogenic MYC expression through super-enhancer-mediated gene gating Nat. Genet. 2019-12-01 [PMID: 31784729] (PLA, Human)
• Lewandowska Ronnegren A Crosstalk between environmental signals and 3d genome organization in the regulation of gene expression Thesis 2021-01-01 (ChIP)
• Chachoua I, Tzelepis I, Dai H et al. Canonical WNT signaling-dependent gating of MYC requires a noncanonical CTCF function at a distal binding site Nature communications 2022-01-11 [PMID: 35017527] (ChIP, PLA, Human)
• Cavazza T, Takeda Y, Politi AZ et al. Parental genome unification is highly error-prone in mammalian embryos Cell 2021-05-27 [PMID: 33964210] (IHC, Bovine, Human)
  
  Details:
  Immunocytochemistry was performed using the ELYS antibody on human and sheep zygotes. These immunofluorescent images revealed the location of during meiosis.

Bioinformatics

• Gene Symbol: AHCTF1