eIF5A Antibody

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Western Blot: eIF5A Antibody [NBP1-51982] - Analysis of eIF5A in MOLT4 lysate (35ug protein in RIPA buffer) using NBP1-51982 at 0.1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated

Order Details

eIF5A Antibody Summary

Immunogen
Peptide with sequence C-RPPHRASFLKRLESK corresponding to internal region (near N-Terminus) according to NP_001137232.1.
Epitope
C-RPPHRASFLKRLESK
Specificity
Reported variants NP_001137233.1, NP_001961.1 and NP_001137234.1 represent identical protein:
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EIF5A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 3 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 20 kDa band observed in MOLT4 lysates (calculated MW of 20.2 kDa band according to NP_001137232.1).
Publications
Read Publication using NBP1-51982.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for eIF5A Antibody

  • eIF4D
  • eIF-4D
  • eIF5A
  • EIF-5A
  • EIF5A1
  • eIF-5A1
  • eIF-5A-1
  • eIF5AI
  • Eukaryotic initiation factor 5A isoform 1
  • eukaryotic initiation factor 5A
  • eukaryotic translation initiation factor 5A
  • eukaryotic translation initiation factor 5A-1
  • MGC104255
  • MGC99547
  • Rev-binding Factor

Background

EIF5A, previously known as EIF4D and IF-M2Ba, is a highly conserved 17-kDa protein that was originally characterized as a translation initiation factor, promoting the formation of the first peptide bond in mRNA translation in vitro. This is best positioned as a factor that controls the balance between cellular proliferation and death, depending upon the nature of cellular stress. Moreover, mutational analysis using human EIF5A to complement an EIF5A null strain of S. cerevisiae supports a role for EIF5A in protein synthesis. The activity of EIF5A is modulated by the covalent addition of the spermidine derivative hypusine to lysine residue 50.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for eIF5A Antibody (NBP1-51982)(1)


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol EIF5A
Entrez
Uniprot