eIF5A Antibody (8C1) - Azide and BSA Free

Western Blot: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of EIF5A expression in transfected 293T cell line by EIF5A monoclonal antibody (M01), clone 8C1.Lane 1: EIF5A transfected lysate(16.8 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: eIF5A Antibody (8C1) [H00001984-M01] - Analysis of monoclonal antibody to EIF5A on HeLa cell . Antibody concentration 10 ug/ml.

Sandwich ELISA: eIF5A Antibody (8C1) [H00001984-M01] - Detection limit for recombinant GST tagged EIF5A is 3 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 8C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

eIF5A Antibody (8C1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: EIF5A (NP_001961.1, 1 a.a. ~ 154 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADDLDFETGDAGASATFPMQCSALRKNGFVVLKGRPCKIVEMSTSKTGKHGHAKVHLVGIDIFTGKKYEDICPSTHNMDVPNIKRNDFQLIGIQDGYLSLLQDSGEVREDLRLPEGDLGKEIEQKYDCGEEILITVLSAMTEEAAVAIKAMAK
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EIF5A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Sandwich ELISA
  • Western Blot
• Application Notes: It has been used for ELISA and WB.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eIF5A Antibody (8C1) - Azide and BSA Free

• eIF4D
• eIF-4D
• eIF5A
• EIF-5A
• EIF5A1
• eIF-5A1
• eIF-5A-1
• eIF5AI
• Eukaryotic initiation factor 5A isoform 1
• eukaryotic initiation factor 5A
• eukaryotic translation initiation factor 5A
• eukaryotic translation initiation factor 5A-1
• MGC104255
• MGC99547
• Rev-binding Factor

Background

The human eukaryotic translation initiation factor 5A (eIF5A) is an 18 kDa protein. eIF5A precursor [(ec-eIF-5A(lys)] is the only cellular protein known to contain a specific lysine residue which is transformed into the unique amino acid hypusine [N-(4-amino-2-hydroxybutyl)-lysine] by a series of post translational reactions. eIF5A promotes the formation of the first peptide bond during the initial stage of protein synthesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF5A
• Uniprot:
  • NP_001961(Human)