eIF4H Antibody

Western Blot: eIF4H Antibody [NBP1-00105] - Whole cell lysate from HeLa (5, 15 and 50 ug) and 293T (T; 50 ug) cells. Antibody used at 0.04 ug/ml.

Immunohistochemistry-Paraffin: eIF4H Antibody [NBP1-00105] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-eIF4H used at a dilution of1:200 (1ug/ml). Detection: DAB

• Reactivity: Hu, Mu, Rt, Pm-Or
• Applications: WB, IHC, IP (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

eIF4H Antibody Summary

• Description: Novus Biologicals Rabbit eIF4H Antibody (NBP1-00105) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 198 and 248 of human eukaryotic translation initiation factor 4H using the numbering given in entry NP_071496.1 (GeneID 7458).
• Predicted Species: Mouse (100%), Rat (100%), Orangutan (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4H
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Western Blot 1:2000- 1:10000
• Application Notes: It is not recommended for immunoprecipitation. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Control:

  eIF4H Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for eIF4H Antibody

• eukaryotic translation initiation factor 4H
• KIAA0038eIF-4H
• WBSCR1
• Williams-Beuren syndrome chromosome region 1
• WSCR1Williams-Beuren syndrome chromosomal region 1 protein

Background

This gene encodes one of the translation initiation factors, which functions to stimulate the initiation of protein synthesis at the level of mRNA utilization. This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene generates 2 transcript variants. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EIF4H
• Entrez:
  • 7458(Human)
• Uniprot:
  • NP_071496.1(Human)