eIF4GII Overexpression Lysate

Images

 
Western Blot: eIF4GII Overexpression Lysate (Adult Normal) [NBL1-10211] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for eIF4GII.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

eIF4GII Overexpression Lysate Summary

Description
Transient overexpression lysate of eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3) The lysate was created in HEK293T cells, using Plasmid ID RC212591 and based on accession number NM_003760. The protein contains a C-MYC/DDK Tag.
Gene
EIF4G3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
176.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for eIF4GII Overexpression Lysate

  • eIF-4G 3
  • eIF-4-gamma 3
  • eIF-4-gamma II
  • eIF4GIIeIF4G 3
  • eukaryotic translation initiation factor 4 gamma 3
  • eukaryotic translation initiation factor 4 gamma, 3

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-268
Species: Hu, Po
Applications: WB, IP
NB100-81898
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NB120-11929
Species: Hu, Mu
Applications: WB, Flow
NBP2-24529
Species: Hu, Mu, Ca, Ch, Pm
Applications: WB, Simple Western, IHC, IHC-P
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P, KO
MAB208
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NB100-56708
Species: Hu, Mu, Rt, Po, Ch, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, HEStain, IB, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready, IHC-FrFl, KD, KO
NBP1-49535
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-24632
Species: Hu, Mu, Rt, Ca, Ch, Pm
Applications: WB, IHC, IHC-P
NB120-6125
Species: Hu, Bv, Ca, Pm, Xp, Dr(-), Mu(-)
Applications: WB, ELISA, Flow, ICC/IF, IP, MiAr, CyTOF-ready
NBP1-57310
Species: Hu
Applications: WB, IHC, IHC-P
NBP1-77685
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-32870
Species: Hu, Ch
Applications: WB, IHC, IHC-P, IP, KD
NBP2-37488
Species: Hu, Mu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
AF7899
Species: Hu
Applications: WB, IHC
NB100-56116
Species: Hu, Mu, Rt, Ma
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NBP2-45511
Species: Hu, Mu, Rt
Applications: WB, IHC

Publications for eIF4GII Lysate (NBL1-10211) (0)

There are no publications for eIF4GII Lysate (NBL1-10211).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for eIF4GII Lysate (NBL1-10211) (0)

There are no reviews for eIF4GII Lysate (NBL1-10211). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for eIF4GII Lysate (NBL1-10211) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBL1-10211

Additional eIF4GII Products

Bioinformatics Tool for eIF4GII Lysate (NBL1-10211)

Discover related pathways, diseases and genes to eIF4GII Lysate (NBL1-10211). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for eIF4GII Lysate (NBL1-10211)

Discover more about diseases related to eIF4GII Lysate (NBL1-10211).
 

Pathways for eIF4GII Lysate (NBL1-10211)

View related products by pathway.

PTMs for eIF4GII Lysate (NBL1-10211)

Learn more about PTMs related to eIF4GII Lysate (NBL1-10211).

Blogs on eIF4GII

There are no specific blogs for eIF4GII, but you can read our latest blog posts.

Customers Who Bought This Also Bought

eIF4GII Antibody
NBP2-16309

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our eIF4GII Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol EIF4G3