eIF4ENIF1 Antibody - Azide and BSA Free

Western Blot: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of EIF4ENIF1 expression in Jurkat.

Immunocytochemistry/Immunofluorescence: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of purified antibody to EIF4ENIF1 on HeLa cell. (antibody concentration 10 ug/ml)

Western Blot: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of EIF4ENIF1 expression in Hela S3 NE.

Western Blot: eIF4ENIF1 Antibody [H00056478-B01P] - Analysis of EIF4ENIF1 expression in transfected 293T cell line by EIF4ENIF1 polyclonal antibody. Lane 1: EIF4ENIF1 transfected lysate(108.35 KDa). Lane 2: Non-transfected lysate.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IP, Dual ISH-IHC
• Clonality: Polyclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

eIF4ENIF1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse eIF4ENIF1 Antibody - Azide and BSA Free (H00056478-B01P) is a polyclonal antibody validated for use in WB, Dual RNAscope ISH-IHC, ICC/IF and IP. Anti-eIF4ENIF1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: EIF4ENIF1 (AAH33028.1, 1 a.a. - 985 a.a.) full-length human protein. MDRRSMGETESGDAFLDLKKPPASKCPHRYTKEELLDIKELPHSKQRPSCLSEKYDSDGVWDPEKWHASLYPASGRSSPVESLKKELDTDRPSLVRRIVDPRERVKEDDLDVVLSPQRRSFGGGCHVTAAVSSRRSGSPLEKDSDGLRLLGGRRIGSGRIISARTFEKDHRLSDKDLRDLRDRDRERDFKDKRFRREFGDSKRVFGERRRNDSYTEEEPEWFSAGPTSQSETIELTGFDDKILEEDHKGRKRTRRRTASVKEGIVECNGGVAEEDEVEVILAQEPAADQEVPRDAVLPEQSPGDFDFNEFFNLDKVPCLASMIEDVLGEGSVSASRFSRWFSNPSRSGSRSSSLGSTPHEELERLAGLEQAILSPGQNSGNYFAPIPLEDHAENKVDILEMLQKAKVDLKPLLSSLSANKEKLKESSHSGVVLSVEEVEAGLKGLKVDQQVKNSTPFMAEHLEETLSAVTNNRQLKKDGDMTAFNKLVSTMKASGTLPSQPKVSRNLESHLMSPAEIPGQPVPKNILQELLGQPVQRPASSNLLSGLMGSLEPTTSLLGQRAPSPPLSQVFQTRAASADYLRPRIPSPIGFTPGPQQLLGDPFQGMRKPMSPITAQMSQLELQQAALEGLALPHDLAVQAANFYQPGFGKPQVDRTRDGFRNRQQRVTKSPAPVHRGNSSSPAPAASITSMLSPSFTPTSVIRKMYESKEKSKEEPASGKAALGDSKEDTQKASEENLLSSSSVPSADRDSSPTTNSKLSALQRSSCSTPLSQANRYTKEQDYRPKATGRKTPTLASPVPTTPFLRPVHQVPLVPHVPMVRPAHRLHPGLVQRMLAQGVHPQHLPSLLQTGVLPPGMDLSHLQGISGPILGQPFYPLPAASHPLLNPRPGTPLHLAMVQQQLQRSVLHPPGSGSHAAAVSVQTTPQNVPSRSGLPHMHSQLEHRPSQRSSSPVGLAKWFGSDVLQQPLPSMPAKVISVDELEYRQ
• Specificity: Reacts with eukaryotic translation initiation factor 4E nuclear import factor 1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Mouse
• Gene: EIF4ENIF1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Dual RNAscope ISH-IHC
  • Immunocytochemistry/ Immunofluorescence
  • Immunoprecipitation
  • Western Blot 1:500
• Application Notes: This antibody is reactive against cell and transfected lysate in western blot, immunofluorescence, and as a detection antibody in ELISA. Dual ISH-IHC validation (PMID: 29395907).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29395907).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for eIF4ENIF1 Antibody - Azide and BSA Free

• 4E-TFLJ26551,2610509L04Rik
• Clast4
• eIF4E-transporter
• eukaryotic translation initiation factor 4E nuclear import factor 1eIF4E transporter
• eukaryotic translation initiation factor 4E transporter
• FLJ21601

Background

The protein encoded by this gene is a nucleocytoplasmic shuttle protein for the translation initiation factor eIF4E. This shuttle protein interacts with the importin alpha-beta complex to mediate nuclear import of eIF4E. It is predominantly cytoplasmic;its own nuclear import is regulated by a nuclear localization signal and nuclear export signals. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for eIF4ENIF1 Antibody (H00056478-B01P)(3)

Publications using H00056478-B01P

• Zahr SK, Yang G, Kazan H, A Translational Repression Complex in Developing Mammalian Neural Stem Cells that Regulates Neuronal Specification. Neuron. 2018-02-07 [PMID: 29395907] (Dual ISH-IHC, Mouse)
• Amadei G, Zander MA, Yang G et al. A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis. J Neurosci. 2015-11-25 [PMID: 26609159]
• Yang G, Smibert CA, Kaplan DR, Miller FD. An eIF4E1/4E-T Complex Determines the Genesis of Neurons from Precursors by Translationally Repressing a Proneurogenic Transcription Program Neuron. 2014-11-06 [PMID: 25456498]

Bioinformatics

• Gene Symbol: EIF4ENIF1
• Entrez:
  • 56478(Human)
• Uniprot:
  • AAH33028.1(Human)