eIF4E Antibody (163CT48.1.9)

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Product Details

Summary
Product Discontinued
View other related eIF4E Primary Antibodies

Order Details


    • Catalog Number
      NBP2-29968
    • Availability
      Product Discontinued

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eIF4E Antibody (163CT48.1.9) Summary

Immunogen
This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
Localization
Cellular Location Cytoplasm, P-body.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
EIF4E
Purity
Protein G purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:10-50
  • Western Blot 1:100 - 1:500
Theoretical MW
25.097 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.09% Sodium Azide
Purity
Protein G purified

Alternate Names for eIF4E Antibody (163CT48.1.9)

  • CBP
  • eIF4E
  • eIF-4E
  • EIF4E1
  • EIF4EL1
  • EIF4EL1MGC111573
  • eIF-4F 25 kDa subunit
  • EIF4F
  • eukaryotic translation initiation factor 4E
  • eukaryotic translation initiation factor 4E-like 1
  • mRNA cap-binding protein

Background

EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol EIF4E
Uniprot