eIF4B Antibody

Western Blot: eIF4B Antibody [NBP2-16306] - A. 30 ug 293T whole cell extract B. 30 ug whole cell extract of human EIF4B-transfected 293T cells 7.5 % SDS-PAGE EIF4B antibody dilution: 1:5000

Immunocytochemistry/Immunofluorescence: eIF4B Antibody [NBP2-16306] - Analysis of paraformaldehyde-fixed MCF-7, using antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: eIF4B Antibody [NBP2-16306] - Paraffin-embedded mouse kidney. EIF4B antibody [N1N2], N-term dilution: 1:500.

Western Blot: eIF4B Antibody [NBP2-16306] - Sample (30 ug of whole cell lysate) A: NT2D1 B: IMR32 C: U87-MG D: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.

Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EIF4B antibody diluted by 1:1000.

Western Blot: eIF4B Antibody [NBP2-16306] - Various whole cell extracts (30 ug) were separated by 7.5 % SDS-PAGE, and blotted with EIF4B antibody diluted by 1:1000

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

eIF4B Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human eIF4B. The exact sequence is proprietary.
• Predicted Species: Rat (97%), Rhesus Macaque (100%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF4B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:10000
• Theoretical MW: 69 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for eIF4B Antibody

• Eif4a2
• eIF4B
• EIF-4B
• eukaryotic translation initiation factor 4B
• PRO1843

Background

Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: EIF4B
• Uniprot:
  • P23588()