EIF3B Antibody

Western Blot: EIF3B Antibody [NBP2-24571] - analysis of eIF3B in A431 cell lysate in the 1) absence and 2) presence of immunizing peptide and 3) NIH 3T3 cell lysate using eIF3B antibody at 0.25 ug/ml, 0.25 ug/ml and 0.05 ug/ml respectively.

Immunohistochemistry-Paraffin: EIF3B Antibody [NBP2-24571] - Formalin-fixed, paraffin-embedded human brain tissue stained with eIF3B antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Simple Western: EIF3B Antibody [NBP2-24571] - Simple Western lane view shows a specific band for EIF3B in 0.5 mg/ml of A431 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Discontinued

EIF3B Antibody Summary

• Immunogen: A portion of amino acids 1-50 of human eIF3B was used as the immunogen for the antibody.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EIF3B
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Simple Western 1:80
  • Western Blot 0.05-0.25 ug/ml
• Application Notes: In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.

Reactivity Notes

Reactive in human and mouse. Predicted to cross react with primate samples by homology.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.2 ml PBS and 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EIF3B Antibody

• eIF3 p110
• eIF3 p116
• eIF3b
• eIF-3-eta
• EIF3-P110
• EIF3-P116
• EIF3S9EIF3-ETA
• Eukaryotic translation initiation factor 3 subunit 9
• eukaryotic translation initiation factor 3 subunit B
• eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD)
• eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa
• eukaryotic translation initiation factor 3, subunit B
• MGC104664
• MGC131875
• Prt1 homolog
• PRT1hPrt1

Background

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EIF3B Antibody (NBP2-24571)(1)

Publication using NBP2-24571

• Shin S, Han MJ, Jedrychowski MP et al. mTOR inhibition reprograms cellular proteostasis by regulating eIF3D-mediated selective mRNA translation and promotes cell phenotype switching Cell Reports 2023-08-01 [PMID: 37494188] (WB, Human)
  
  Details:
  Supplementary figure 5

Bioinformatics

• Gene Symbol: EIF3B
• Uniprot:
  • P55884(Human)