EHD4 Antibody - BSA Free

Western Blot: EHD4 Antibody [NBP3-35385] - Western blot analysis of various lysates using EHD4 Rabbit pAb at 1:3000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

EHD4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EHD4 Antibody - BSA Free (NBP3-35385) is a polyclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 362-541 of human EHD4 (NP_644670.1).
  
  Sequence:
  MQEQLENYDFTKFHSLKPKLIEAVDNMLSNKISPLMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVAKDKPVYDELFYTLSPINGKISGVNAKKEMVTSKLPNSVLGKIWKLADCDCDGMLDEEEFALAKHLIKIKLDGYELPSSLPPHLVPPSHRKSLPKAD
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHD4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for EHD4 Antibody - BSA Free

• EH domain containing 4
• EH domain-containing protein 4
• EH-domain containing 4
• HCA10
• HCA11
• Hepatocellular carcinoma-associated protein 10/11
• hepatocellular carcinoma-associated protein HCA11
• ortholog of rat pincher
• PAST homolog 4
• PAST4

Background

EHD4 is an EH domain containing protein expressed in the extracellular matrix. EHD4 is the first extracellular matrix protein described that contains an EH domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EHD4