EHD3 Antibody

Western Blot: EHD3 Antibody [NBP3-12300] - 1:500 andibody dilution in DiluObuffer. Apparent MW is 70 KDa.

Immunohistochemistry-Paraffin: EHD3 Antibody [NBP3-12300] - Mouse kidney. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 40X magnfication.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

EHD3 Antibody Summary

• Description: Novus Biologicals Rabbit EHD3 Antibody (NBP3-12300) is a polyclonal antibody validated for use in IHC, WB, ELISA and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide taken within amino acid region 475-535 on human EH-domain containing protein 3.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EHD3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunohistochemistry 1:50-1:150
  • Immunohistochemistry-Paraffin 1:50-1:150
  • Immunoprecipitation 1:200
  • Western Blot 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for EHD3 Antibody

• EH domain containing 3
• EH domain-containing protein 3
• EHD2
• EH-domain containing 3
• PAST homolog 3
• PAST3

Background

The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EHD3