EHD2 Antibody (2D8)

Western Blot: EHD2 Antibody (2D8) [H00030846-M01] - EHD2 monoclonal antibody (M01), clone 2D8 Analysis of EHD2 expression in HeLa.

Immunocytochemistry/Immunofluorescence: EHD2 Antibody (2D8) [H00030846-M01] - Analysis of monoclonal antibody to EHD2 on HeLa cell. Antibody concentration 10 ug/ml.

Western Blot: EHD2 Antibody (2D8) [H00030846-M01] - Analysis of EHD2 expression in transfected 293T cell line by EHD2 monoclonal antibody (M01), clone 2D8.Lane 1: EHD2 transfected lysate(61.2 KDa).Lane 2: Non-transfected lysate.

Sandwich ELISA: EHD2 Antibody (2D8) [H00030846-M01] - Detection limit for recombinant GST tagged EHD2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF
• Clone: 2D8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

EHD2 Antibody (2D8) Summary

• Immunogen: EHD2 (NP_055416, 365 a.a. ~ 427 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. AHDFTKFHSLKPKLLEALDEMLTHDIAKLMPLLRQEELESTEVGVQGGAFEGTHMGPFVERGP
• Localization: Nuclear
• Specificity: EHD2 - EH-domain containing 2
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: EHD2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Reactivity Notes

Human. Other species not tested.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for EHD2 Antibody (2D8)

• EH domain containing 2
• EH domain-containing protein 2
• EH-domain containing 2
• FLJ96617
• PAST homolog 2
• PAST2

Background

This gene encodes a member of the EH domain-containing protein family. These proteins are characterized by a C-terminal EF-hand domain, a nucleotide-binding consensus site at the N terminus and a bipartite nuclear localization signal. The encoded protein interacts with the actin cytoskeleton through an N-terminal domain and also binds to an EH domain-binding protein through the C-terminal EH domain. This interaction appears to connect clathrin-dependent endocytosis to actin, suggesting that this gene product participates in the endocytic pathway.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: EHD2
• Entrez:
  • 30846(Human)
• OMIM:
  • 605890(Human)
• Uniprot:
  • NP_055416(Human)